Results 126–150 of 211 for "pcr."
-
Pseudorabies in a group of Texas hog-hunting dogs
...ncephalitis with lesions consistent with viral infection. The pseudorabies PCR results were positive. Pseudorabies is caused by suid herpesvirus 1. This virus has been eradicated from the commercial swine population in the United States, but the virus still circulates in feral swine populations. The prevalence of pseudorabies in Texas feral swine populations averages between 8-13% based on opportunistic sampling of adults done by the USDA/APHIS/Wi...
-
How to create a comprehensive serology testing plan
...uation? Example: Following a positive serology test for leptospirosis, a rtPCR test on urine may hopefully identify and confirm the cause of the animal’s illness. Example: Following a positive serology test for Johne’s Disease, a rtPCR test on feces may yield a negative result because the organism is shed intermittently, even though the animal actually has Johne’s Disease. If a serology screening yields a negative result for antibodies to an agent...
-
Texas Animal Health Commission updates trichomoniasis testing requirements
...amples must arrive at laboratory within 48 hours of collection In-Pouch (rtPCR) Ship in In-Pouch Incubate samples at 37ºC (98.6ºF) for 48 hours prior to testing Samples must arrive at laboratory within 96 hours of collection Pooling eligible for up to five samples Direct Smegma (rtPCR) Ship in tube containing 1.5 mL of PBS or sterile saline Samples must arrive at laboratory within 120 hours of collection Samples must be chilled from collection unt...
-
TVMDL detects feline calicivirus in multi-cat household
...sis even with treatment (Dodd, J. personal communication, April 24, 2018). PCR technology can be used to identify oral calicivirus carriers. If you have any questions about this case or TVMDL’s Feline Calicivirus qPCR test please call Dr. Cheryl Maguire, veterinary diagnostician at 979-845-3414. For more information about TVMDL’s test catalog, visit tvmdl.tamu.edu. References 1. Gracis M. Inflammatory Oral Diseases in Cats. World Small Animal Asso...
-
Epizootic hemorrhagic disease (EHD) in White-Tailed deer
...ical, hyperacute, acute, or chronic. EHDV nucleic acid can be detected by qPCR or EHDV can be isolated in the subclinical form, but clinical signs and macroscopic and microscopic lesions are absent and death does not occur. In the hyperacute form, sudden death is the outcome. Swelling of the head, neck, tongue, and eyelids can be observed in the acute form. In this form, there is fever, depression, anorexia, weakness, respiratory distress, ptyalis...
-
Tick-Borne Relapsing Fever diagnosed in Brazos Valley dog
...along with a visibly decreased platelet count (Figure 1). Borrelia spp. rtPCR was then run and DNA from B. turicatae was detected with the cycle thresholds (Ct) being 21.6. According to TVMDL’s molecular laboratory, samples with a Ct value 1.0-28.0 indicate the presence of abundant amounts of target nucleic acid; samples with a Ct value of 28.1-35.0 indicate moderate amounts of target nucleic acid; samples with a Ct value of 35.1-40-45.0 indicate...
-
Diagnostic Guidance: Postmortem Atlanto-occipital Cerebral Spinal Fluid Sampling in Large Animals
...Referral], Additive free (red top) sample collection tube: Microprotein, Electrolyte Profile, Bacterial Identification – Companion Animal (Aerobic & Anaerobic Culture) or Bacterial Identification – Livestock (Aerobic & Anaerobic Culture), Listeria monocytogenes (PCR) [Referral], West Nile Virus (rtPCR), Equine West Nile Virus IgM (ELISA), Eastern Equine Encephalitis Virus IgM (ELISA), Equine Encephalitis Panel (ELISA), Western Equine Encephalitis...
-
Detection of Neospora caninum DNA in a mummified bovine fetus
...tozoa and viruses by standard methods. For the last two decades, real time PCR (rtPCR) has been the pillar in the diagnosis of infectious abortion in ruminants and it is of particular value in mummified fetuses where other ancillary tests are inadequate. References de la Concha-Bermejillo A, Romano JE: Pregnancy loss in cattle. Clinical Theriogenology; 2021, Submitted. de la Concha-Bermejillo A, Romano JE: The use of laboratory in pregnancy loss....
-
Tick-borne disease in a dog with no known history of ticks
...ne Relapsing Fever, which was later confirmed with the TickPath Layerplex qPCR test at TVMDL. In the state of Texas, Tick-Borne Relapsing Fever (TBRF) is caused by Borrelia turicatae, which is spread by Ornithodoros turicata, a soft-bodied tick. These ticks are nocturnal, feeding on their hosts briefly at night. Therefore, it is not uncommon for dogs diagnosed with TBRF to lack a clinical history of tick infestations. The infection can cause letha...
-
Mast Cell Tumor Prognostic Panel [Referral]
Evaluation of the biological behavior of a specific tumor entity. Includes IHC C-Kit, IHC Ki-67, PCR C-Kit Exon 8, PCR C-Kit Exon 11, and Agnor Special Staining.
-
TVMDL retires Canine Distemper Virus (FA)
...e College Station laboratory at 1.888.646.5623....
-
Testing Options for Anaplasma marginale in Cattle
...right-Giemsa stain, as well as Diff-Quick®, can be utilized for staining. qPCR (real time polymerase chain reaction) testing utilizes EDTA whole blood or fresh tissues to look for the DNA of the Anaplasma qPCR is best at detecting acute infections when bacteremia is still present, but may still be able to detect some infections after visual identification of the organism is no longer present on a blood smear. cELISA (competitive ELISA) testing is...
-
Fetal loss and neonatal mortality due to Neospora caninum in a litter of German Shorthaired Pointers
...e not observed but fresh liver was tested via Canine Herpesvirus Type 1 (rtPCR) and CHV was not detected. Histology demonstrated necrotizing encephalitis, lymphoplasmacytic myocarditis, rhabdomyositis and pneumonia with protozoan cyst in the brain and intralesional tachyzoites in several tissues. These findings were consistent with systemic protozoal disease. Neospora caninum was detected in brain/heart tissue pool via (rtPCR) with a Ct Value of 2...
-
Diagnostics of a disaster: Animal disease concerns during floods
...orms a microscopic agglutination test (MAT) and polymerase chain reaction (PCR) test for leptospirosis. The MAT can provide titers that indicate infection in the presence of compatible clinical signs. The PCR test can detect the presence of the organism in clinical specimens, which helps differentiate an infected animal from one that may be showing vaccine titers. Clostridial Diseases Herds can be vaccinated to protect against clostridial diseases...
-
TVMDL pathologist contributes to three studies over cetacean pathology
...perspective of an unusual mortality event in Guiana dolphins, a real-time PCR to detect Cetacean morbillivirus, and histopathological differential diagnoses of Meningoencephalitis. Access each article at the links below. The Pathology of Cetacean Morbillivirus Infection and Comorbidities in Guiana Dolphins During an Unusual Mortality Event (Brazil, 2017–2018) A novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceans Histopathol...
-
Cryptosporidium in a Gerenuk
...hoid hyperplasia, which was likely in response to the enteritis. Real-time PCR for cryptosporidium was used to confirm presence of cryptosporidium in feces of the calf. Cryptosporidium is a protozoal infection that affects a wide range of species (mammalian, avian, reptilian and piscine hosts) in addition to being zoonotic and affecting man. Animals obtain the protozoa through exposure to oocysts shed in the feces. Unapparent infections are common...
-
Cryptosporidiosis in cattle
...hology), bacterial culture and sensitivity, and polymerase chain reaction (PCR) testing for viruses. There were no gross findings reported on the submittal form by the veterinarian. On microscopic examination, the major finding was the lesion in the small intestine. This lesion consisted of inflammation that was composed of a mixed-inflammatory cell population including macrophages, lymphocytes, plasma cells and neutrophils that were primarily wit...
-
Tracking transboundary and emerging diseases: Newly funded project aims to use Next-Generation Sequencing to detect bovine diseases sooner
...thogen characterization. Currently, real-time polymerase chain reaction (rtPCR) assays are most widely used in diagnostic laboratories for rapid pathogen detection. These assays are useful, however, they are based on existing genomic information and may fail to detect emerging pathogens or variants of known pathogens. Additionally, rtPCR assays do not yield specific genomic information needed to track the source of disease outbreaks and differenti...
-
TVMDL retires Mycoplasma gallisepticum (HI) and Mycoplasma synoviae (HI) tests
...tive, clients may request Mycoplasma gallisepticum & synoviae Multiplex (rtPCR). The rtPCR test is more sensitive and specific than the HI tests. Clients who still wish to use the HI method may submit samples to Poultry Research and Diagnostic Laboratory (PRDL) at Mississippi State University or the Georgia Poultry Laboratory Network (GPLN). For more information on TVMDL’s test offerings, visit tvmdl.tamu.edu or call one of the agency’s four labor...
-
Direct smegma samples now accepted for trichomonas testing at TVMDL’s Canyon lab
...stic Laboratory (TVMDL) has expanded the Tritrichomonas foetus – Bovine (rtPCR) test offering. Clients interested in testing bovine samples for Tritrichomonas foetus can now submit direct smegma samples to the Canyon laboratory. Direct smegma samples should be submitted in a test tube with 1.5 mL of phosphate buffered saline and chilled during transit. To ensure samples arrive in a testable condition, smegma should be submitted to TVMDL within 96...
-
Diagnostic guidance: Mycoplasma testing options
...nfertility, miscarriages, and weak offspring. These bacteria can also affect the mammary glands causing mastitis and poor milking. Though Mycoplasma species need special conditions to grow, TVMDL can culture for the bacteria from tissues or swabs. There are also polymerase chain reaction (PCR) tests that target the bacteria’s nucleic acid (DNA). Some of the PCR tests are specific for certain Mycoplasma species. Antibody tests are also available fo...
-
Ehrlichia canis discovered in dog
...observed. Based on this finding, TVMDL’s Tick-Borne Pathogen Multiplex (rtPCR) test was performed and confirmed the presence of Ehrlichia canis. Ehrlichiosis in dogs is most commonly caused by infections with Ehrlichia canis and Ehrlichia ewingii. In the acute phases of the disease, morulae can be observed within WBCs during blood smear examination. Morulae are cytoplasmic vacuoles filled with bacteria. E. canis can be spread by the brown dog tic...
-
Acute Toxoplasma gondii Infection Confirmed in a Canine
...tic Laboratory (TVMDL) in College Station for Toxoplasma gondii testing (rtPCR) which was positive with a Ct value of 15.70. Additional testing included Toxoplasma gondii IgG & IgM Panel IFA and Neospora caninum IFA on serum. IgG antibodies against Toxoplasma gondii were detected at a dilution of >=1:2048. IgM antibodies were not detected; nor were Neospora caninum antibodies detected. Toxoplasma gondii tachyzoites may be detected in various tissu...
-
Histopathologic Diagnosis of Feline Herpesvirus
...aboratory (TVMDL). The most common laboratory methods of diagnosis include PCR and histopathology, while exposure to the virus can be demonstrated by measuring antibody response with the virus neutralization assay. This summer, two feline submissions demonstrated an array of respiratory histopathologic lesions that can be seen with herpesvirus infection. These cases were received two days apart but were completely unrelated and from two different...
-
Díaz-Delgado publishes two articles over marine mammal pathology
...with Toxoplasma gondii. Immunohistochemical and polymerase chain reaction (PCR) analysis targeting a repetitive 529 bp DNA fragment of T. gondii confirmed toxoplasmosis in the liver, spleen, lung and lymph nodes. PCR-restriction fragment length polymorphism (RFLP) analysis using 11 markers identified a new non-archetypal genotype, ToxoDB-RFLP genotype #300. Further, the genotyping by microsatellite technique employed 15 markers and confirmed a uni...