Results 151–175 of 211 for "pcr."
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Diagnostic guidance: Tick-Borne Relapsing Fever
...blood films is considered diagnostic for TBRF in dogs. TVMDL also offers a PCR for TBRF Borrelia spp. Test: Borrelia spp. (rtPCR) Turnaround: This test is performed at the College Station laboratory with results in 1 to 3 business days. Price: $30 Treatment TBRF has been successfully treated in dogs with varying doses of doxycycline. On average, 6 mg/kg doxycycline by mouth every 12 hours for a minimum of three weeks appears to resolve clinical si...
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Babesia gibsoni (IFA) temporarily unavailable
...ho suspect Babesia gibsoni in canines may consider TVMDL’s Babesia spp. (rtPCR) as an alternative. Please note that clients testing for export purposes should review the country of interest’s export requirements to confirm PCR is an accepted testing method. If serologic testing is more appropriate, TVMDL can forward samples to Protatek Reference Laboratory in Mesa, Arizona. TVMDL is working with the slide manufacturer and will reorder more once th...
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Education Library: Bovine
...Keeping cattle healthy and hydrated during a drought BVDV: Sources and Test Guidance Definitive Diagnosis of Cryptosporidiosis Photosensitization: affecting cattle herds across the state Testing for Trichomonas Submitting Samples for Trich rtPCR Collecting samples for bovine trichomoniasis testing (video) How to submit samples for trich testing (video) Bovine Syndromic Diagnostic Plans Bovine Testing Guidance and Reference Material...
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Phelue Anderson, MS, PhD
...Dr. Anderson completed his doctorate in Poultry Science at Texas A&M University; previously he received a Master of Science in Animal Science from Prairie View A&M University. His graduate research focused on the use of PCR-based methodologies to characterize Salmonella serotypes of poultry origin. He is interested in bacteriology, molecular and serology testing of poultry diseases, as well as quality assurance programs for diagnostic work...
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West Nile Virus & Encephalitis
...ncephalitis Virus (EEE) with one confirmed case of WNV (brain sample by rt-PCR), 15 confirmed cases of WNV by IgM ELISA and one confirmed case of EEE by IgM ELISA. It is important to remember that these diseases have a seasonality. Our highest number of positive cases are usually observed in September and October (see figure 1.). There is still time to have your horse vaccinated for these preventable diseases. Mosquito control around your barn, st...
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IMHA in dogs secondary to Babesia gibsoni diagnosed at TVMDL
...consistent with Babesia gibsoni (see image), which were later confirmed by PCR. Babesia gibsoni is most commonly observed in pit bull terriers and greyhounds and infections are reported in several states throughout the US. IMHA is suspected when compatible CBC findings are observed, such as a marked regenerative anemia, hemoglobin:HCT mismatch, macroagglutination, and leukocytosis. Evaluation of a blood smear at a diagnostic laboratory is an essen...
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TVMDL Sections
...of both viral and bacterial pathogens using the polymerase chain reaction (PCR), which does not require culturing pathogens, thus eliminating the need to isolate or culture the pathogen, making the test results available quicker. A limited number of tests designed to detect genetic defects are also offered. The Necropsy Section performs necropsies on deceased animals to determine a probable cause of death or to collect specimens for additional tes...
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Infectious laryngotracheitis diagnosed in laying chicken
...large numbers of lymphocytes and plasma cells. Polymerase chain reaction (PCR) evaluation of tracheal swabs was positive for infectious laryngotracheitis virus. Infectious laryngotracheitis (ILT) is a respiratory tract infection of chickens caused by Gallid herpesvirus type 1 (GaHV-1). The virus may cause severe production losses due to mortality and/or decreased egg production. Severe epizootic forms of infection are characterized by signs of re...
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Cattle herds recently diagnosed with Johne’s Disease
...ease as an initial diagnosis. Follow up testing using the Johne’s Disease qPCR resulted in the detection of Mycobacterium avium subspecies paratuberculosis. This bacterium is the causative agent of Johne’s Disease, which was determined to the be primary cause of the weight loss and diarrhea in both cattle herds. In addition to testing for Johne’s Disease, a complete blood count and chemistry panel were requested for one case. Identification of Myc...
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Rhinosporidium diagnosed in Texas horses
...f diagnosis include cytology of nasal exudate to detect sporangiospores or PCR testing on fresh tissue. Currently, only histopathology and cytology are offered at the TVMDL. To learn more about this case, contact Dr. Gabriel Gomez, veterinary pathologist at the College Station laboratory. For more information on TVMDL’s test catalog, visit tvmdl.tamu.edu. Nasal Epithelium. The epithelium contains a pocket of inflammatory cells comprised primarily...
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Bovine CBC reveals concurrent BLV and Anaplasmosis
...sms consistent with Anaplasma marginale. Confirmatory testing included BLV PCR and Anaplasmosis ELISA. Bovine leukemia virus (BLV) is a retrovirus spread by contact with bodily fluids through biting flies, breeding, colostrum/milk, and certain management practices. Infections can results in asymptomatic carriers or severe clinical disease. Approximately 40% of infected cattle will develop persistent lymphocytosis, as seen in this case. Although nu...
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Neonatal Calf Health Issues
...ollowing tests routinely performed at TVMDL – bacterial culture, serology, PCR, virus isolation, and histopathology. Neonatal calf health issues can be complex and besides infectious disease or nutritional problems, may involve deficiencies in herd management. For this reason, please take advantage of TVMDL’s Veterinary Services for consultation on disease and management related conditions. In the area of livestock, Dr. Guy Sheppard at the College...
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Bacillary hemoglobinuria in feeder cattle
...iagnostic Laboratory (TVMDL) for culture, histopathology, Leptospira spp. qPCR, and histotoxic clostridium FA. Culture of the liver and kidney was negative for pathologic organisms. Histopathology revealed mild to moderate chronic fibrosing portal hepatitis with intralesional fluke eggs and adult flukes. Liver histopathology also showed acute centrilobular to midzonal hepatic necrosis with bile stasis. Molecular diagnostics on the urine, kidney, a...
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Strongylus vulgaris associated with liver damage and neurologic symptoms in a gelding
...re from fecal samples, a laborious and time consuming process. A real-time PCR assay has been developed and validated for the detection and semi-quantification of S. vulgaris eggs in fecal samples. Even more recently reported is the isolation of a S. vulgaris antigen, SvSXP, which may be used to detect targeted IgG antibodies against the parasite during the prepatent period. While generally viewed as a “historical” parasite, veterinarians should b...
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Abortion of a sheep fetus due to Cache Valley Fever Virus
...vities. Because of the type of lesions identified at the gross necropsy, a PCR test for Cache Valley Fever was performed with a positive result. The Cache Valley virus is in the order Bunyaviridae and genus Orthobunyavirus. It was first isolated from mosquitoes collected in Utah’s Cache Valley. This virus can cause outbreaks of spontaneous abortion and congenital malformations in sheep. In a non-pregnant adult animal, an infection may result in a...
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High mortality in a litter of puppies: Infection with canine herpesvirus
...chymotic renal hemorrhage. Available confirmatory diagnostic tests include PCR and histopathology, though viral inclusions are sometimes difficult to find histologically. Figure 1: Photo of kidney lesions at the time of necropsy. These kidneys have disseminated petechial to ecchymotic hemorrhages, a characteristic lesion of canine herpesvirus infection. Figure 2: Photomicrograph depicting renal necrosis (pink area in center) and hemorrhage. Inset:...
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Bacillary hemoglobinuria in a cow
...toxic clostridial specieswas positive for Clostridium novyi/hemolyticum. AqPCR test forLeptospirawas negative. Microscopic lesions in the liver consisted ofmultifocal to confluent areasof severe, acute coagulation necrosis characterized by hepatocyte swelling, cytoplasmic hypereosinophilia, karryorhexis,and pyknosis. The areas of hepatic necrosis were surrounded by a thick rim of degenerate neutrophils and necrotic debris. The gross and microscopi...
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Toxoplasmosis in a Herd of Kangaroos
...nd, most commonly in the heart and rarely in the brain and lung. T. gondii PCR was performed on the heart and brain from one kangaroo and was positive. This toxoplasmosis outbreak had a high mortality rate and left few survivors in the herd. Kangaroos and wallabies are highly susceptible to systemic toxoplasmosis and outbreaks can be devastating. In these species, infected animals often have sudden death though they may show neurological or respir...
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Cytoxic necrotizing factor 1 gene (CNF1) detected in E. coli isolated from a kitten lung
...was exudative and necrotizing, subacute, and severe, indicating a possible bacterial infection. Bacterial culture of the lung resulted in heavy growth of Escherichia coli. No other organisms were isolated. The cytotoxic necrotizing factor 1 gene (CNF1) was detected in this isolate by PCR testing. The significance of this finding remains unclear as there are only a few case reports discussing CNF1 detected in E. coli isolated from cats and dogs. F...
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Lymphoma diagnosed in a dog within an hour of fine needle aspiration via digital cytology service
...When the cytologic findings are equivocal, stained slides can be used for PCR for antigen receptor rearrangement (PARR). PARR testing can often confirm the diagnosis of lymphoma when cytologic examination is concerning but not definitive. For more information about this case, contact Clinical Pathology Section Head, Dr. Julie Piccione. To learn more about TVMDL’s testing services, visit tvmdl.tamu.edu or call 1.888.645.5623. Figure 1. Photomicrog...
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Specialty Services
...he art testing in areas such as mass spectrometry, classical and real-time PCR, nucleic acid sequencing, and rapid bacterial identification. We maintain a focus on developing and validating new diagnostic testing technologies and platforms to ensure our capabilities remain first class. Nontraditional Species TVMDL counts some of the largest wildlife preserves, zoos, and speciality animal operations as its clients. Staff are skilled in assisting wi...
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Ulcerative Enteritis discovered in bobwhite quail
...revealed no evidence of coccidia or nematodes. Polymerase chain reaction (PCR) testing of intestinal and liver samples demonstrated the presence of Clostridium colinum,the cause of ulcerative enteritis. Ulcerative enteritis is an acute bacterial infection of quail, but it also occurs in young chickens, turkeys, and other upland game birds. It is transmitted via the fecal-oral route under natural conditions; birds become infected by ingesting cont...
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Canine Enteropathogen Panel [Referral]
Canine panel includes PCR testing for Clostridium perfringens enterotoxin gene, net F toxin gene-C. perfringens, C. difficile, Campylobacter jejuni and coli, canine parvovirus, Salmonella spp., and IFA testing for Giardia and Cryptosporidium.
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Feline Enteropathogen Panel [Referral]
Feline panel includes includes PCR testing for Clostridium perfringens enterotoxin gene, net F toxin gene-C. perfringens, C. difficile, Campylobacter jejuni and coli, feline panleukopenia virus (FPV), Salmonella spp., Tritrichomonas foetus, and IFA testing for Giardia and Cryptosporidium.
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Unusual Manifestation of Papular Stomatitis in a Feedlot Steer
...) in the cell culture was compatible with bovine popular stomatitis virus. PCR analysis of viral nucleic acid was confirmatory for bovine papular stomatitis virus. Papular stomatitis is usually a mild disease in cattle and occurs worldwide. It is associated with proliferative skin lesions in the absence of systemic clinical disease and is most commonly seen as single or multiple cases in young cattle although outbreaks in dairy cattle are reported...