Results 101–125 of 210 for "pcr."
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Equine Syndromic Diagnostic Plans
...r Brain) CSF, aborted material, brain or heart tissue - Referral UCDavis rtPCR - EEE PCR (CSF or Brain) Brain tissue - Referral NVSL - Sacrocaudalis dorsalis muscle biopsy Click here for sample collection instructions - Referral NMDL-MSU - Botulism ABC rtPCR Feces, feed - Referral U of Penn - Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Equine Infectious Abortion Diagnostic Plan This plan was created to assist with...
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Bovine Syndromic Diagnostic Plans
...e) pure isolate 1 day Bacteriology CAN CS MTWRF MTWRF Moraxella speciation PCR pure isolate - Referral KSVDL - IBK qPCR Panel (Moraxella bovoculi Moraxella bovis, Mycoplasma bovoculi, Mycoplasma bovis and bovine herpesvirus 1) eye swab - Referral KSVDL - Mycoplasma culture – Livestock charcoal swab in transport media 14 days Bacteriology CAN CS MTWRFSa MTWRFSa Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Bovine Ser...
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Neurologic Disease due to Bovine Herpesvirus-5 (BHV-5) Infection in a 3-week-old Charolais mix calf
...ar skin by rtPCR and Neospora caninum DNA was not detected in brain tissue PCR by rtPCR. Given that the changes observed in the brain were highly suggestive of a viral infection, additional testing was performed in consultation with the submitter. A bovine herpesvirus rtPCR test that detects subtypes 1 and 5 was positive (Ct value 28.5) in the brain tissue of this animal. A gel-based PCR assay that differentiates the subtypes confirmed infection w...
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Collaborative project leads to improved diagnostic detection of strangles
...individually. Although quicker and more sensitive than bacterial culture, PCR testing for strangles is still somewhat limited. PCR testing uses primers to target specific DNA sequences to determine an organism’s presence. Because of the genetic similarities between S. equi and S. zoo, some tests may not be able to differentiate between the two organisms and lead to false positives. Conversely, there are instances where the typically targeted sequ...
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TVMDL adds panel to test catalog to aid in the detection of strangles
...Multiple sample types can be submitted for this panel for testing by both PCR and culture. When submitting swabs, it is recommended to send duplicate swabs, one for PCR and a separate one for culture. Swabs submitted in bacterial transport media e.g., gel, Ames charcoal, etc. are not suitable for PCR testing due to inhibitory effects of the media. Clients are strongly encouraged to review the sample types and sampling requirements listed for this...
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Summary of Calf Diarrhea Multiplex Results from Texas Cattle, 2019-2023
...ailable epidemiological data from Texas cattle tested using this multiplex PCR. Animal Demographics & Geographic Locations Over the 4.5-year period for which data is available (March 2019 – October 2023), TVMDL performed 1,088 calf diarrhea multiplex PCR tests. Samples submitted for this test originated from 108 different counties in Texas. A dot density map representing the geographic distribution of samples is below; each dot represents a single...
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Bovine Abortion Caused by Leptospira spp.
...the organism in the serum from the cow or through antigen detection (e.g. PCR, rtPCR, etc.) testing of fetal tissues. The organism is shed in the urine of infected animals, thus personal protective equipment (PPE) should be utilized when working with cattle suspected to be infected in order to prevent human exposure. Additionally, minimizing exposure of susceptible animals to wildlife, rodents, and contaminated water sources, along with vaccinati...
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Q fever abortion diagnosed at TVMDL
...all small ruminant abortions submitted to TVMDL be tested for Q fever by qPCR before any other test can be performed. If C. burnetiiq PCR results are positive, Select Agent regulations require that all fresh and fixed tissues must be destroyed properly and no other testing can be performed. Coxiella burnetii primarily affects goats, sheep, cattle, and humans. Dogs and cats can be infected by ingesting placentas and tissues of infected aborted fet...
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Bovine Abortion Diagnostics find BVD
...not detected via PCR. Bovine viral diarrhea virus (BVDV) was detected via PCR of the fetal tissues with a Ct value of 18.70. The cutoff Ct value for a positive BVDV PCR sample is <37.00. Bovine Comprehensive Abortion Serology was requested on the dam serum sample at the start of testing as well. Antibody testing was negative for Brucella abortus, Histophilus somni, Neospora caninum, and 5 serovars of Leptospira spp. There was a low (8) virus neut...
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Avian metapneumovirus (aMPV) tests added to TVMDL test catalog
...ay with results in 1 to 4 business days. Avian Metapneumovirus Type C (rRT-PCR) Test Code: 1612 Price: $28 Turnaround Time: This test is performed Monday through Friday with results in 1 to 4 business days. Avian Metapneumovirus Panel (rRT-PCR) Test Code: 1613 Price: $65 Turnaround Time: This test is performed Monday through Friday with results in 1 to 4 business days. For more information on TVMDL’s molecular diagnostics test offerings, visit tvm...
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Lyme Disease Testing at TVMDL
...es (Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, Anaplasma phagocytophilum); Borrelia species (B. burgdorferi, B. hermsii, B. parkeri, B. turicatae); and Babesia species (B. gibsoni, B. canis, B. caballi). Test: Borrelia species (qPCR) Price: $30 Test: TickPath LayerPlex (qPCR) Price: $60 Borrelia burgdorferi depicted using IFA To learn more about TVMDL’s serology tests, contact Sandy Rodgers, Serology Section Head. For more...
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Porcine Circovirus type 2: A focus on respiratory disease
...t TVMDL to aid in the diagnosis of PCV-2 include Necropsy, Histopathology, PCR, virus isolation, and Serology. The recommended samples for PCR include intestine, fecal swab, lung or fetal tissue. For serology, an ELISA test is available, and serum is the required sample. To learn more about this case, contact Dr. Gabriel Gomez, veterinary pathologist, Dr. Pam Ferro, virology and molecular diagnostics section head in College Station, or Dr. Binu Ve...
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Swine Influenza Virus: A focus on the respiratory syndrome
...seen in the regenerative stage of the disease. For a definitive diagnosis, PCR on fresh tissue (trachea, lung, tracheal swab), serology (serum), immunohistochemistry on fixed tissue and virus isolation on fresh lung are tests available to help confirm an SIV infection. TVMDL currently offers necropsies and microscopic examination, virus isolation, serology, and PCR testing on fresh tissue. To learn more about this case or about Swine Influenza Vir...
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Listeriosis in a goat doe
...stically rewarding when submitted for cytology or pathogen detection via rtPCR if brain tissue is not available. Culture and rtPCR of CSF is less often rewarding compared to appropriate sections of affected fresh brain tissue. Figure 1: Photomicrograph depicting necrosuppurative encephalitis with microabscessation in the brainstem of the goat. Resources: Allen, Andrew L., Brad A. Goupil, and Beth A. Valentine. “A Retrospective Study of Brain Lesio...
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Epidemiology of feline hemoparasites in Texas
...ented as having 2 other “adult littermates” with similar symptoms. 17 cats were diagnosed by observing haemofelis in peripheral blood smears. One of these cats was also PCR positive. 15 cases were identified by PCR; 2 cats were still PCR positive 1 month after the initial diagnosis. To learn more about TVMDL’s feline hemoparasite testing, and other offerings, visit tvmdl.tamu.edu or call 1.888.646.5623....
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Infectious respiratory disease in a group of dogs
...dogs. In addition, canine parainfluenza virus type-2 RNA was detected by rtPCR (Ct values ranged from 24.5 to 29.5) in the lungs of the three dogs. Canine distemper virus, influenza A virus, canine adenovirus type 2, canine coronavirus, canine herpesvirus type 1 and Bordetella bronchiseptica nucleic acid were not detected by PCR in any of the dogs. Pneumonia in dogs is part of the canine infectious respiratory disease complex (CIRDC) and is a mult...
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TVMDL adds test to detect causative agent of ulcerative enteritis in avian species
...m bacteria are difficult to isolate with traditional culture methods, this PCR test allows for improved detection of the agent to aid in diagnosis of this disease. Test: Clostridium colinum (rtPCR) Test Code: 1556 Turnaround Time: This test is performed Tuesday through Friday at the College Station laboratory with results in one to four business days. Price: $25 For more information about TVMDL testing, visit tvmdl.tamu.edu or call one of the agen...
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Download Fee Schedule
...cine Parvovirus (HI) [Referral] 1 mL serum $12.21 8168 Porcine Parvovirus (PCR) [Referral] fetal tissue $35.00 8169 Porcine Parvovirus Type 2 (PCR) [Referral] One of the following: serum lung spleen $35.00 1519 Porcine Reproductive & Respiratory Syndrome Virus (ELISA) 1 mL serum $7.50 Canyon 1309 Porcine Reproductive & Respiratory Syndrome Virus (rtPCR) One or more of the following: 1-2 mL oral fluid, semen, serum 1-2 g fresh lung or fetal tissu...
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Small Animal Syndromic Diagnostic Plans
...tests TestSamplesTurnaround TimeSectionLabSchedule Campylobacter jejuni (rtPCR) 1-2 grams feces 1-3 days Molecular Diagnostics CAN CS TWRF Salmonella genus (rtPCR) 1-2 grams feces 1-4 days Molecular Diagnostics CS TWRF Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Feline Diarrhea Diagnostic Plan This plan was created to assist with the investigation of etiologic agents that may cause disease in cats. Recommended ini...
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Equine Protozoal Myeloencephalitis (EPM) Diagnosed in a Horse
...of the disease, though additional testing such as immunohistochemistry or PCR may be necessary to confirm the diagnosis. Rare cases of EPM are also caused by Neospora hughesi, which cannot be reliably differentiated histologically from Sarcocystis neurona. Immunohistochemistry and/or PCR are needed for ultimate differentiation. Besides EPM, there are many other differentials for neurologic disease in horses. One disease that should always be rule...
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Porcine Reproductive and Respiratory Syndrome: A focus on the respiratory syndrome
...boratory to aid in the diagnosis of PRRS include Necropsy, Histopathology, PCR, virus isolation, and Serology. The recommended samples for PCR testing include oral fluid, semen, serum, EDTA whole unclotted blood, lung, or fetal tissue. For serology, an ELISA and IFA test are available and serum is the required sample. To learn more about this case, contact Dr. Gomez, Veterinary Pathologist, Dr. Ferro, Microbiology Branch Chief, at the College Stat...
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CHI St. Joseph Health and Texas A&M Veterinary Medical Diagnostic Laboratory Collaborate for COVID-19 Testing
...) test, one of the two tests that are currently used to test for COVID-19. PCR swabs are currently collected at all CHI St. Joseph Health Emergency Centers as well as the South College Station Respiratory Clinic. Through this partnership, CHI St. Joseph Health staff can test up to 500 COVID-19 samples each day, allowing St. Joseph to greatly increase the number of tests performed daily in the community. “TVMDL is dedicated to protecting both anima...
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TVMDL, Physician’s Preferred Laboratory, Ltd. establish partnership to increase human COVID-19 testing
...e information needed to manage patient care, community health and safety.” PCR testing is the primary method used to detect COVID-19. PCR tests are used to detect the virus antigens. Antigens indicate the presence of a virus in its early stages of infection, even before antibodies form or symptoms are present. Dr. Andrew Hoot of Amarillo Pathology Group, LLP and Physicians Preferred Laboratory, Ltd. will serve as the medical director overseeing hu...
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Pseudorabies in a group of Texas hog-hunting dogs
...ncephalitis with lesions consistent with viral infection. The pseudorabies PCR results were positive. Pseudorabies is caused by suid herpesvirus 1. This virus has been eradicated from the commercial swine population in the United States, but the virus still circulates in feral swine populations. The prevalence of pseudorabies in Texas feral swine populations averages between 8-13% based on opportunistic sampling of adults done by the USDA/APHIS/Wi...