Tritrichomonas foetus – Bovine (rtPCR)

Detection of Tritrichomonas foetus in bovine samples by real time PCR.

One of the following: 

• inoculated InPouch TF or direct smegma in phosphate buffered saline (PBS) or sterile saline
• 1-2 mL preputial washes from bulls
• 1-2 mL cervical washes from cows
• inoculated trich pouch
• 1-2 mL stomach content from aborted fetus

Per the Texas Animal Health Commission’s (TAHC) regulatory testing requirements, up to 5 samples may be pooled at one time if indicated on the submission form. Clients must submit individual samples and TVMDL must complete pooling. 

TVMDL pools samples for $1.15 per sample, allowing the client to test five samples for the price of one. Pricing example: 5 samples submitted and pooling requested would be charged $28.25 testing fee + $5.75 pooling fee for a total of $34.00 plus accession fee. 

If a pooled sample results in a positive test, TVMDL will identify the positive animal(s) by testing the individual samples in the pool at the regular price of $28.25 per test. Without the individual tests, all bulls in a positive pool are required by the State to go to slaughter. 

• InPouch collection device or sealed, leak-proof plastic bag or container. 
• Samples for direct smegma PCR should be placed in a test tube with 1.5 mL of PBS.

• Label all samples. 
• Maintain and ship InPouch TF samples overnight at ambient temperature. Protect from extreme heat and cold. 
• Maintain direct smegma in test tube at refrigerator temperature. Ship overnight on cold packs. Protect from extreme heat and cold.

Texas Animal Health Commission (TAHC) requirements must be met for this to be considered an official test by TAHC. See the TAHC website for details.This is a test for a disease agent regulated by the Texas Animal Health Commission (TAHC) and/or the United States Department of Agriculture’s Animal and Plant Health Inspection Service (USDA APHIS). Samples, documentation, and testing may be subject to additional requirements or restrictions as determined by TAHC or USDA APHIS.