Rabies Virus Antibody Endpoint Titer (RFFIT) [Referral]

Referral tests are not performed at TVMDL; a processing and shipping fee will apply.

Measurement of antibodies to rabies virus by rapid fluorescent foci inhibition test (RFFIT).

2 mL serum

Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). When possible, transfer clear serum off the clot into a new tube. Excessively hemolyzed samples are not acceptable for this test.

Glass or plastic tube with no anticoagulant additives.

Label all samples with unique identifiers. 

For multiple samples, number tubes consecutively using a permanent marker or adhesive label. Include a list of sample names/numbers or the Excel specimen import spreadsheet with submittal form. Place the tubes in consecutive, numerical order in cardboard boxes designated for tubes. Do not submit loose in bags. 

Use cold packs during hot weather, especially if shipping serum samples on the clot. Overnight shipping is recommended.

This test is performed at the Kansas State University Rabies Laboratory in Manhattan, KS. Prices listed for tests performed outside TVMDL may not include referral lab accession fees and are subject to change without notice. A processing and shipping fee is added to all referral tests. Turnaround times vary depending on shipping time and the referral lab’s schedule.

Rabies screen and titer tests listed are performed by the RFFIT method. The FAVN test is required by many rabies-free countries or regions in order for dogs and cats to qualify for a reduced quarantine period prior to entry. The FAVN test has a different price than the RFFIT and export paperwork requires a veterinarian’s signature. If you require FAVN testing for export purposes, send the sample directly to the Kansas State University Rabies Laboratory for the most expedient results. 

For assistance on problems or questions regarding rabies prevention, call your local health department, the Texas Department of State Health Services (DSHS), your DSHS Public Health Regional Office, DSHS Zoonosis Control Branch (512-776-7255), or the DSHS Infectious Disease Control Unit (512-776-7455).