Avian Influenza Matrix (rtPCR)

Detection of influenza A virus (IAV) by real time PCR.

One or more of the following: 

• tracheal swab
• oropharyngeal swab
• cloacal swab
• 1-2 mL tracheal wash 
• 1 g fresh trachea

Review sample collection recommendations from the USDA National Veterinary Services Laboratory to ensure adequate and accurate samples. 

Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid, and discard the swab. Brain heart infusion (BHI) broth or viral transport media are preferred; saline may be used if necessary. 

Use synthetic or semi-synthetic swabs (e.g., polyester, rayon, nylon) with a plastic handle (flocked or spun head).  Avoid cotton or calcium alginate swabs or swabs with wooden handles which have been shown to inactivate virus and inhibit PCR.

BHI broth or viral transport media in a sealed, leak-proof bag or container.

Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.

This is a test for a disease agent regulated by the Texas Animal Health Commission (TAHC) and/or the United States Department of Agriculture’s Animal and Plant Health Inspection Service (USDA APHIS). Samples, documentation, and testing may be subject to additional requirements or restrictions as determined by TAHC or USDA APHIS.