One or more of the following: 1-2 mL semen, 1-2 mL EDTA whole blood, 1 g fresh spleen.
Blood in heparin (green top) tube cannot be used for this test. Ensure thorough mixing of blood in EDTA (e.g. invert tube several times immediately following collection) to prevent clotting. Clotted blood is not suitable for testing. Fresh samples provide the most accurate results.