Vitamin E (HPLC)

10 g liver or 2 mL serum.

Serum samples with hemolysis visually estimated at over 100 mg/dL Hb will not be assayed for vitamins A or E. A useful tool for estimating hemolysis level may be found at https://www.cdc.gov/ncezid/dvbd/pdf/Hemolysis_Palette_Bookmark-P.pdf .  Protect samples from light and ship to stay cold. 

Liver: sealed, leak-proof plastic bag or container. Do not submit liver in formalin.

Serum: plastic or glass tube with no anticoagulant additives.  Remove serum from red cells and place in an additive free transport tube.

Label all samples. Ship overnight on cold packs.

TVMDL is in the process of transitioning vitamin assays from HPLC to LC-MS/MS. During this transition period, samples for vitamin A and E analysis will be forwarded to the Michigan State University Veterinary Diagnostic Laboratory in Lansing, MI each Monday. Results are expected within 7-10 business days after delivery to Michigan State.