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Texas A&M Veterinary Medical Diagnostic Laboratory

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Chronic Wasting Disease (IHC)

Detection of abnormal prion protein, the suspected causative agent of chronic wasting disease, in either antemortem samples (biopsies of retropharyngeal lymph node, tonsil, or rectal wall), or postmortem samples (retropharyngeal lymph node or obex) by immunohistochemical staining.

NameChronic Wasting Disease (IHC)
Code1232
SectionHistopathology
SpeciesCervid
Specimen

Antemortem: biopsy of the retropharyngeal lymph node, tonsils, or rectal wall in 10% neutral buffered formalin (NBF). 

Postmortem: one of the following: retropharyngeal lymph node, obex, or entire brain in 10% neutral buffered formalin (NBF). Entire head also accepted.

Sampling Requirements

Fresh obex and retropharyngeal lymph node are also accepted.

Collection Container

Fixed samples: sealed, leak-proof, wide mouth plastic container. Samples submitted in glass containers are not acceptable and will incur an additional processing charge. 

Entire head: chill to refrigeration temperatures before packaging. Wrap in a plastic bag, preferably large zipper lock bags or leak-proof bags securely sealed with packing tape. Place inside second leak-proof plastic bag, with sufficient absorbent material and freezer packs. Place inside third bag and tape shut. Place paperwork in a separate, sealed plastic bag.

Packing Instructions

Label all samples. Ship fixed tissue at ambient temperature. Ship fresh tissue overnight on cold packs. Place paperwork and the head in a shipping container. The outer container should be insulated and crush-proof. Large plastic picnic coolers are ideal and do not need to be placed inside a cardboard box for shipping. Cardboard boxes without rigid coolers are not appropriate as condensation causes them to fall apart in shipping

Special Instructions

Turnaround times vary due to the testing surge associated with the TPWD mandatory CWD testing requirements. 

If entire heads are submitted, TVMDL will collect obex and retropharyngeal lymph nodes for an additional charge of $21.50/per animal; a $21.50/animal head disposal fee will also apply. 

Include the TWIMS submission form which can be accessed through your Texas Parks and Wildlife Department TWIMS account.

Price$48.50
Schedule
Lab Schedule Turnaround time
College Station Monday, Tuesday, Wednesday, Thursday, Friday days

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