Vitamin A (HPLC)

10 g liver or 2 mL serum

Serum samples with hemolysis visually estimated at over 100 mg/dL Hb will not be assayed for vitamins A or E. A useful tool for estimating hemolysis level may be found at https://www.cdc.gov/ncezid/dvbd/pdf/Hemolysis_Palette_Bookmark-P.pdf .  

Protect samples from light and ship to stay cold. 

Do not submit liver in formalin. Remove serum from red cells and place in an additive free transport tube.

Liver: sealed, leak-proof plastic bag or container. 

Serum: plastic or glass tube with no anticoagulant additives.

Label all samples. Ship overnight on cold packs. Samples should be protected from light immediately following collection.

TVMDL is transitioning vitamin assays from HPLC to LC-MS/MS. During this transition period, samples for vitamin A and E analysis will be forwarded to the Michigan State University Veterinary Diagnostic Laboratory in Lansing, MI each Monday. Results are expected within 7-10 business days after delivery to Michigan State.