InPouch Trich samples or direct smegma in PBS (phophate buffered saline)
1-2 mL preputial washes from bulls, cervical washes from cows, inoculated trich pouch, or stomach content of aborted fetus. Samples for direct smegma PCR should be placed in a test tube with 1.5 mL of PBS.
Trich pouch, leak proof plastic container, plastic or glass tube
InPouch: Label all samples. Maintain and ship samples overnight at room temperature. Protect from extreme heat and cold.
Direct Smegma qPCR: Label all samples. Maintain at refrigerator temperature and ship overnight n cold packs. Protect from extreme heat and cold.
Up to 5 properly collected and handled samples may be pooled for testing.
Samples must be collected and shipped individually to TVMDL. For Texas Animal Health Commission's regulatory testing requirements, up to 5 samples may be pooled and pooling must be completed at TVMDL
TVMDL pools samples for $1 per pouch, allowing the client to test five samples for the price of one. Pricing example: 5 samples submitted and pooling requested would be charged $25 testing fee + $5 pooling for a total of $30 plus accession fee.
If a pooled sample results in a positive test, TVMDL will identify the positive animal(s) by testing the individual samples in the pool at the regular price of $25 per test. Without the individual tests, all bulls in a positive pool are required by the State to go to slaughter.
Pooling is not available for samples using the Direct smegma qPCR method. Direct smegma qPCR is only performed at the College Station laboratory.