InPouch Trich samples; Direct smegma in PBS (phophate buffered saline)
1-2 ml preputial washes from bulls, cervical washes from cows, inoculated trich pouch, or stomach content of aborted foetus. Samples for Direct smegma PCR should be placed in a test tube with 1.5 mL of PBS.
Trich pouch, leak proof plastic container, plastic or glass tube
InPouch: Label all samples. Maintain and ship samples at room temperature. Protect from extreme heat and cold. Ship overnight.
Direct Smegma qPCR: Label all samples. Maintain at refrigerator temperature and ship on cold packs. Protect from extreme heat and cold. Ship overnight.
POOLING SAMPLES TO REDUCE COSTS
Up to 5 properly collected and handled samples may be officially pooled for testing.
Samples must be collected and shipped individually to the laboratory; TVMDL will pool samples at the lab for $1 per pouch if pooled testing is requested. For official TAHC testing, up to 5 samples may be pooled and pooling must be completed at the TVMDL. The client will essentially be testing five samples for the price of one with pooling. (Pricing example: 5 samples submitted and pooling requested would be charged $25 testing fee + $5 pooling for a total of $30 plus accession fee.)
If a pooled sample results in a positive test, TVMDL will identify the positive animal(s) by testing the individual samples in the pool at the regular price of $25 per test. Without the individual tests, all bulls in a positive pool are required by the State to go to slaughter. Pooling is not available for samples using the Direct smegma qPCR method. Direct smegma qPCR is only ran at the College Station laboratory.