These sample requirements are for endogenous ACTH only. If performing an ACTH stimulation test, please see the specific ACTH stimulation test for sampling requirements.
--Draw blood and place in a EDTA blood tube. --Spin within one hour of collection. --Remove EDTA plasma. --Place at least 0.5 mL of EDTA plasma in a plastic tube free of any additives (i.e. don't just remove LitHep donut), then place the tube in a secondary container (i.e. specimen cup) with water, freeze sample in container filled with water. --ACTH is a fragile hormone and needs to be frozen on arrival and in plastic for best results.
Label all samples. Ship overnight on cold packs or dry ice so sample arrives frozen.