Detection of 5 pathogens commonly attributed to bovine respiratory disease; includes bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVD), bovine parainfluenza-3 (PI-3), infectious bovine rhinotracheitis/bovine herpesvirus type 1 (BHV-1), and bovine coronavirus (BCOV) all by real time PCR.