Detection of abnormal prion protein, the suspected causative agent of scrapie, in both retropharyngeal lymph node and obex via immunohistochemical staining.
Specimen
Antemortem: biopsy of the eyelid or rectal wall in 10% neutral buffered formalin (NBF).Postmortem: retropharyngeal lymph nodes, obex, or entire brain in 10% neutral buffered formalin (NBF). The entire head is also accepted. Certification for export of semen or embryos: the whole body must be submitted for collection and testing of brain, brainstem, spinal cord, palatine tonsils, spleen, mesenteric and retropharyngeal lymph nodes, and distal ileum.
Measurement of selenium by inductively-coupled plasma/mass spectrometry. A $10.75 sample preparation fee will be added to tissue and feed specimens.
Specimen
One or more of the following:
1 mL EDTA whole blood
5 g fresh liver
50 mg fresh liver biopsy (at least 3 Tru-Cut samples, no fluid added)
2 mL serum or plasma
500 g feed
For birds, 0.6 mL EDTA whole blood is adequate.
Blood, serum, and urine samples are tested on Mondays, Wednesdays, and Thursdays with a turnaround time of 7 days. Tissue and feed samples are tested on Mondays and Thursdays with a turnaround time of 10 days.
Detection of antibodies to ovine progressive pneumonia (OPP) virus in ovine, and caprine arthritis encephalitis (CAE) virus in caprine by competitive ELISA.
Measurement of thallium by inductively-coupled plasma/mass spectrometry. A $10.75 sample preparation fee will be added to tissue and feed specimens.
Specimen
One or more of the following:
0.50 mL EDTA whole blood or heparinized blood
5 g fresh liver
50 mg fresh liver biopsy (at least 3 Tru-Cut samples, no fluid added)
500 g feed
5 mL water
For birds, 0.30 mL EDTA whole blood is adequate.
Blood, serum, and urine samples are tested on Mondays, Wednesdays, and Thursdays with a turnaround time of 7 days. Tissue and feed samples are tested on Mondays and Thursdays with a turnaround time of 10 days.