Detection of antibodies to Blastomyces, Coccidioides, Histoplasma, and Aspergillus by agar gel immunodiffusion (AGID); antibodies to Coccidioides, Histoplasma and Aspergillus by complement fixation (CF); and antigen to Cryptococcus neoformans by latex agglutination. Cryptococcus is tested at 1:2 through the endpoint titer; the CF tests are tested at 1:8 through the endpoint titer.