Results 1–209 of 209 for "pcr."
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Types of Positions
...toxins or illicit substances using liquid chromatography–mass spectrometry PCR testing for DNA or RNA analysis Classic bacteriology and fungal cultures ELISA testing for antibody presence Quick turnaround tests for animals in critical status Endocrine testing for pregnancy or breeding purposes Administrative Support Providing excellent client service to our 150,000+ clients is paramount. Administrative staff at all four locations interact with vet...
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Streptococcus equi Multiplex Real-Time PCR and Bacterial Identification Panel
This panel includes detection and differentiation of Streptococcus equi subsp. equi, the causative agent of strangles, and S. equi subsp. zooepidemicus by real-time PCR and identification of these bacterial pathogens by culture.
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Panfungal PCR and Sequencing [Referral]
Identification of fungal organisms by PCR and sequencing. This PCR is recommended to complement identification of fungal organisms on histopathology and cytology. The assay is not recommended when fungal organisms are not observed histologically, due to the potential of amplification of non specific contaminants.
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Porcine Reproductive & Respiratory Syndrome Virus (rtPCR)
Detection and differentiation of North American (NA) and European Union (EU) strains of porcine reproductive and respiratory syndrome virus (PRRS) by real time PCR.
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Bovine Leukemia Virus (rtPCR)
Detection of bovine leukemia virus by real time PCR.
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Bovine Herpesvirus Type 1 (rtPCR)
Detection of bovine herpesvirus type 1, the causative agent of infectious bovine rhinotracheitis (IBR) by real time PCR.
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Equine Coronavirus (rtPCR) [Referral]
Detection of equine enteric coronavirus by PCR.
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Mycoplasma spp. (PCR)
Detection of Mycoplasma species by PCR.
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PCR for Antigen Receptor Rearrangement – Cytology Specimen [Referral]
Distinction of reactive (polyclonal) lymphocytes from neoplastic (monoclonal) lymphocytes by PCR.This assay is recommended for cytology specimens and fluid only; for fixed tissue samples see TVMDL referral test #8177, PCR for Antigen Receptor Rearrangement – Fixed Tissue [Referral].
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PCR for Antigen Receptor Rearrangement – Fixed Tissue [Referral]
Differentiation of a monoclonal/oligoclonal or neoplastic lymphoid proliferation from a reactive and polyclonal or pseudoclonal proliferation by PCR. This assay is recommended for fixed tissue only; for fluid samples see TVMDL referral test #8022, PCR for Antigen Receptor Rearrangement – Cytology Specimen [Referral].
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Avian Adenovirus (PCR)
Confirmation/identification of avian adenovirus in suspect viral isolates by PCR.
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Eastern Equine Encephalitis Virus (PCR) [Referral]
Detection of eastern equine encephalitis virus by PCR.
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Porcine Parvovirus (PCR) [Referral]
Detection of parvovirus in porcine species by PCR.
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Porcine Parvovirus Type 2 (PCR) [Referral]
Detection of parvovirus in porcine species by PCR.
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Respiratory Disease Panel – Feline (PCR)
Detection of pathogens commonly attributed to respiratory disease in felines; includes bordetella bronchiseptica, feline herpesvirus, feline calicivirus, influenza A, Chlamydophila spp., and Mycoplasma spp., all by real time PCR.
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E. coli Toxin Typing – Companion Animal & Equine (rtPCR)
Detection of E. coli toxin and virulence genes commonly associated with pathogenic strains of E. coli: F4/K88 fimbrial adhesins, STa enterotoxin, LT enterotoxin, CNF1 and CNF2 cytotoxic necrotizing factors, Stx1and Stx2 Shiga like toxins, and eaeA intimin in E. coli isolates by real-time PCR (rtPCR).
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E. coli Toxin Typing – Porcine (rtPCR)
Detection of E. coli toxin and virulence genes commonly associated with pathogenic strains of E. coli: F4/K88 fimbrial adhesins, F5/K99, F6/987P, and F18 fimbrial adhesins and LT enterotoxin in E. coli isolates by real-time PCR (rtPCR).
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E. coli Toxin Typing – Ruminant (rtPCR)
Detection of E. coli toxin and virulence genes commonly associated with pathogenic strains of E. coli: F5/K99 fimbrial adhesins, STa enterotoxin, cs31a adhesins, CNF1 and CNF2 cytotoxic necrotizing factors, Stx1and Stx2 Shiga like toxins, and eaeA intimin in E. coli isolates by real-time PCR (rtPCR).
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Listeria monocytogenes (PCR) [Referral]
Detection of Listeria bacteria by PCR.
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Equine Parvovirus (PCR) [Referral]
Detection of equine parvovirus by PCR.
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Avian Metapneumovirus Type A (rRT-PCR)
Detection of avian metapneumovirus type A by rRT-PCR.
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Avian Metapneumovirus Type B (rRT-PCR)
Detection of avian metapneumovirus type B by rRT-PCR.
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Avian Metapneumovirus Type C (rRT-PCR)
Detection of avian metapneumovirus type C by rRT-PCR.
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Avian Metapneumovirus Panel (rRT-PCR)
Detection of avian metapneumovirus types A, B, and C by rRT-PCR.
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Chlamydia spp. (rtPCR)
Detection of Chlamydia spp. by real time PCR.
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Lawsonia Intracellularis (rtPCR)
Detection of Lawsonia intracellularis by reverse transcription PCR.
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Mycoplasma bovis (rtPCR)
Detection of Mycoplasma bovis by real time PCR.
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Bovine Respiratory Syncytial Virus (rtPCR)
Detection of bovine respiratory syncytial virus (BRSV) by real time PCR.
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Bovine Viral Diarrhea Virus (rtPCR)
Detection of bovine viral diarrhea virus (BVDV) by real time PCR.
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Canine Distemper Virus (rtPCR)
Detection of canine distemper virus (CDV) by real time PCR.
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Equine Herpesvirus Type 1 (rtPCR)
Detection of equine herpesvirus (EHV-1) and differentiation between neuropathogenic and non-neuropathogenic / respiratory / wild type strains by real time PCR.
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Feline Herpesvirus Type 1 (rtPCR)
Detection of feline herpesvirus type 1 (FHV-1) by real time PCR.
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Influenza A Virus Matrix (rtPCR)
Detection of influenza type A (matrix gene), including subtypes H3N8 and H3N2, by real time PCR.
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Infectious Laryngotracheitis Virus (rtPCR)
Detection of infectious laryngotracheitis virus by real time PCR.
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Mycoplasma gallisepticum & synoviae Multiplex (rtPCR)
Detection of Mycoplasma gallisepticum and Mycoplasma synoviae by real time PCR.
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Reticuloendotheliosis Virus (rtPCR)
Detection of reticuloendotheliosis virus (REV) by real time PCR.
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Salmonella genus (rtPCR)
Detection of the Salmonella genus by real time PCR.
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Swine Influenza Virus Matrix (rtPCR)
Detection of the swine influenza virus matrix gene by real time PCR.
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West Nile Virus (rtPCR)
Detection of West Nile virus by real time PCR.
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Tritrichomonas foetus – Bovine (rtPCR)
Detection of Tritrichomonas foetus in bovine samples by real time PCR.
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Borrelia spp. (rtPCR)
Detection of Borrelia species, including B. burgdorferi, B. hermsii, B. parkeri, and B. turicatae by real time PCR.
Lyme Borreliosis (Borrelia burgdorferi) DNA is not typically detected in peripheral blood, even when Lyme disease is the cause of clinical signs.
This PCR for Borrelia species is offered for the detection of Tick-Borne Relapsing Fever Borrelia spp. and for identification of Borrelia burgdorferi in ticks, tissues, and body fluids (joint fluids, CSF, etc.).
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Bovine Coronavirus (rtPCR)
Detection of bovine coronavirus by real time PCR.
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Bovine Influenza D Virus (rtPCR)
Detection of bovine influenza D virus (BIDV) by real time PCR.
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Calf Diarrhea Multiplex (rtPCR)
Detection of the most common viral and protozoal causes of diarrhea in calves 60 days of age and younger. Includes bovine coronavirus, bovine rotavirus A, and cryptosporidium, all by real time PCR.
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Campylobacter jejuni (rtPCR)
Detection of Campylobacter jejuni by real time PCR.
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Canine Herpesvirus Type 1 (rtPCR)
Detection of canine herpesvirus type 1 by real time PCR.
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Equine Herpesvirus Types 1 & 4 (rtPCR)
Detection and differentiation of equine herpesvirus 1 & 4 by real time PCR.
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Feline Calicivirus (rtPCR)
Detection of feline calicivirus (FCV) by real time PCR.
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Giardia (rtPCR)
Detection of giardia by real time PCR.
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Respiratory Disease Panel – Bovine Bacterial (rtPCR)
Detection of 5 pathogens commonly attributed to respiratory disease in bovine; includes Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Arcanobacterium (Truperella) pyogenes, and Mycoplasma bovis, all by real time PCR.
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Respiratory Disease Panel – Canine (rtPCR)
Detection of pathogens commonly attributed to respiratory disease in canines; includes bordetella bronchiseptica, canine adenovirus 2, canine distemper virus, canine herpesvirus 1, canine influenza virus (subtypes H3N8 and H3N2), canine respiratory coronavirus, and canine parainfluenza virus, all by real time PCR.
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Rickettsiales spp. (rtPCR)
Detection of Rickettsiales species, including Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, and Anaplasma phagocytophilum, all by real time PCR.
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Tick-borne Pathogen Panel (rtPCR)
Detection of tick-borne pathogens by real time PCR. Includes Rickettsiales species (Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, Anaplasma phagocytophilum); Borrelia species (B. burgdoreferi, B. hermsii, B. parkeri, B. turicatae); and Babesia species (B. gibsoni, B. canis, B. caballi).
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Toxoplasma gondii (rtPCR)
Detection of Toxoplasma gondii, the causative agent of toxoplasmosis, by real time PCR.
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Neorickettsia risticii (rtPCR) [Referral]
Detection of Neorickettsia risticii (formerly Ehrlichia risticii), the causative agent of Potomac horse fever, by real time PCR.
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Respiratory Disease Panel – Bovine Viral (rtPCR)
Detection of 5 pathogens commonly attributed to bovine respiratory disease; includes bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVD), bovine parainfluenza-3 (PI-3), infectious bovine rhinotracheitis/bovine herpesvirus type 1 (BHV-1), and bovine coronavirus (BCOV) all by real time PCR.
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Avian Reovirus (rtPCR)
Confirmation/identification of avian reovirus in suspect viral isolates by PCR.
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Porcine Circovirus Type 2 (PCR)
Detection of porcine circovirus type 2 by PCR.
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Heterobilharzia americana (PCR) [Referral]
Detection of Heterobilharzia americana, the causative agent of canine schistosomiasis, by PCR.
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C-Kit Mutation (PCR) [Referral]
Detection of exon 8 and exon 11 mutations by PCR to diagnose mast cell tumors.
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Avian Bornavirus (PCR) [Referral]
Detection of avian bornavirus by PCR.
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Hemoplasma (PCR) [Referral]
Detection of Mycoplasma wenyonii and Candidatus Mycoplasma haemobos, the causative agents of hemotropic mycoplasma infection, by PCR.
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Sarcocystis neurona (PCR) [Referral]
Detection of Sarcocystis neurona, the causative agent of equine protozoal myelitis, by PCR.
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Mycoplasma haemolamae (PCR) [Referral]
Detection of Mycoplasma haemolamae by PCR.
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Hepatozoon americanum / canis (PCR) [Referral]
Detection of Hepatozoon americanum / canis by PCR.
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Feline Immunodeficiency Virus (PCR) [Referral]
Detection of feline immunodeficiency virus by PCR.
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Microbiome Dysbiosis Index (PCR) [Referral]
The Dysbiosis Index (DI) is a PCR based assay that quantifies the abundances of 7 bacterial groups and total bacteria and summarizes them into a single number.
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TVMDL retires Bunyavirus and Cache Valley Virus PCR
...The Texas A&M Veterinary Medical Diagnostic Laboratory (TVMDL) has retired the Bunyavirus and Cache Valley Virus PCR due to a decline in test requests. Clients interested in an alternative test option may consider TVMDL’s Cache Valley VN. For more information on TVMDL’s test offerings, visit tvmdl.tamu.edu or call one of the agency’s four laboratory locations....
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Clostridium perfringens Toxin Typing (rtPCR)
Typing of Clostridium perfringens isolates (A, B, C, D, or E) by identifying toxin genes (alpha, cpa; beta, cpb, epsilon, etx; iota, ia) as well as genes for beta 2 toxin (cpb2) and enterotoxin (cpe) by real time PCR.
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Leptospira spp. (rtPCR)
Detection of Leptospira species by real time PCR.
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Avian Influenza Matrix (rtPCR)
Detection of influenza A virus (IAV) by real time PCR.
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Anaplasma marginale (rtPCR)
Detection of Anaplasma marginale by real time PCR.
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Bluetongue Virus (rtPCR)
Detection of bluetongue virus (BTV) by real time PCR.
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Bluetongue Virus Subtyping (rtPCR)
Differentiation between common serotypes of bluetongue virus (serotypes 2, 10, 11, 12, 13 and 17) by real time PCR.
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Duck Enteritis Virus (rtPCR)
Detection of duck enteritis virus by real time PCR.
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Epizootic Hemorrhagic Disease Virus (rtPCR)
Detection of epizootic hemorrhagic disease virus (EHDV) by real time PCR.
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Epizootic Hemorrhagic Disease Virus Subtyping (rtPCR)
Differentiation between common serotypes of epizootic hemorrhagic disease virus (serotypes 1, 2, and 6) by real time PCR.
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Equine Arteritis Virus (rtPCR)
Detection of equine arteritis virus, the causative agent of equine viral arteritis (EVA), by real time PCR.
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G6S Detection (rtPCR)
Detection of the G6S genetic mutation (N-acetylglucosamine-6-sulfatase) by real time PCR.
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Mycobacterium avium subsp. paratuberculosis (rtPCR)
Detection of Mycobacterium avium subspecies paratuberculosis, the causative agent of Johne's disease, by real time PCR. TVMDL provides an option to pool up to three samples if pooling is indicated on the submission form. Pooling will add an additional $2.25 per sample.
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Malignant Catarrhal Fever (rtPCR)
Detection of malignant catarrhal fever (MCF) by real-time PCR. This test can distinguish between the ovine, ibex, white tail deer, caprine and alcelaphine herpesvirus-1 strains of MCF.
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Parvovirus (rtPCR)
Detection of parvovirus in canine or feline samples by real time PCR.
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Coxiella burnetii (rtPCR)
Detection of Coxiella burnetii, the causative agent of Q fever, by real time PCR.
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Tritrichomonas foetus – Feline (rtPCR)
Detection of Tritrichomonas foetus in feline samples by real time PCR.
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Avian Paramyxovirus Type 1 Matrix (rtPCR)
Detection of avian paramyxovirus type 1 by real time PCR.
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Babesia spp. (rtPCR)
Detection of Babesia species (B. gibsoni, B. canis, B. caballi) by real time PCR.
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Cryptosporidium & Giardia Panel (rtPCR)
Detection of cryptosporidium and giardia by real time PCR.
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Cryptosporidium sp. (rtPCR)
Detection of Cryptosporidium sp. by real time PCR.
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Mycoplasma hemofelis (rtPCR)
Detection of Mycoplasma hemofelis, also known as Hemobartonella felis, by real time PCR.
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Neospora caninum (rtPCR)
Detection of Neospora caninum by real time PCR.
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Parapoxvirus (rtPCR)
Detection of parapoxvirus, the causative agent of orf, or contagious echtyma, by real time PCR.
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Porcine Enteric Coronavirus Multiplex (rtPCR)
Detection of porcine enteric coronaviruses including porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis (TGE), and porcine deltacoronavirus (PDCoV), by real time PCR.
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Bovine Viral Diarrhea Virus – Ear Notch (rtPCR)
Detection of bovine viral diarrhea virus (BVDV) by real-time PCR. TVMDL provides an option to pool up to 24 samples if pooling is indicated on the submission form.
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Brachyspira hyodysenteriae/hampsonii (rtPCR) [Referral]
Detection of Brachyspira hyodysenteriae or Brachyspira hampsonii, causative agents of swine dysentery (SD), by real time PCR.
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Severe Acute Respiratory Syndrome Coronavirus 2 (rtPCR)
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease (COVID-19), by real-time PCR.
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Clostridium colinum (rtPCR)
Detection of Clostridium colinum, one of the causative agents of ulcerative enteritis in avian species, by real time PCR.
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Streptococcus equi Multiplex (rtPCR)
Detection and differentiation of Streptococcus equi, subsp. equi, the causative agent of strangles, and S. equi subsp. zooepidemicus by real time PCR.
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Bovine B4 Export Panel (rtPCR)
Detection of 4 pathogens known to infect bovine; includes bovine viral diarrhea virus (BVDV), bovine leukemia virus (BLV), infectious bovine rhinotracheitis/bovine herpesvirus-1 (IBR/ BHV-1), and bluetongue virus (BTV), all by real time PCR.
Testing for this combination of pathogens is often useful in fulfilling certain export requirements.
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Bluetongue & Epizootic Hemorrhagic Disease Virus Panel (rtPCR)
Detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) by multiplex real time PCR.
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Small Ruminant Syndromic Diagnostic Plans
...R) 2 mL EDTA blood 1-4 days Molecular Diagnostics CS TWRF Mycoplasma spp. (PCR) PCR swab 3-7 days Molecular Diagnostics CAN CS WR TR Parapoxvirus (rtPCR) 1 g fresh lesions or scabs 1-4 days Molecular Diagnostics CS TWRF Electron Microscopy 2-5 g fresh samples 5-7 days Virology CS Variable Listeria monocytogenes (PCR) [Referral] 2 mL EDTA blood - Referral Cornell AHDC MTWRF ++Due to the limited number of submissions from some species, validation of...
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Equine Syndromic Diagnostic Plans
...r Brain) CSF, aborted material, brain or heart tissue - Referral UCDavis rtPCR - EEE PCR (CSF or Brain) Brain tissue - Referral NVSL - Sacrocaudalis dorsalis muscle biopsy Click here for sample collection instructions - Referral NMDL-MSU - Botulism ABC rtPCR Feces, feed - Referral U of Penn - Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Equine Infectious Abortion Diagnostic Plan This plan was created to assist with...
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Bovine Syndromic Diagnostic Plans
...e) pure isolate 1 day Bacteriology CAN CS MTWRF MTWRF Moraxella speciation PCR pure isolate - Referral KSVDL - IBK qPCR Panel (Moraxella bovoculi Moraxella bovis, Mycoplasma bovoculi, Mycoplasma bovis and bovine herpesvirus 1) eye swab - Referral KSVDL - Mycoplasma culture – Livestock charcoal swab in transport media 14 days Bacteriology CAN CS MTWRFSa MTWRFSa Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Bovine Ser...
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Neurologic Disease due to Bovine Herpesvirus-5 (BHV-5) Infection in a 3-week-old Charolais mix calf
...ar skin by rtPCR and Neospora caninum DNA was not detected in brain tissue PCR by rtPCR. Given that the changes observed in the brain were highly suggestive of a viral infection, additional testing was performed in consultation with the submitter. A bovine herpesvirus rtPCR test that detects subtypes 1 and 5 was positive (Ct value 28.5) in the brain tissue of this animal. A gel-based PCR assay that differentiates the subtypes confirmed infection w...
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Collaborative project leads to improved diagnostic detection of strangles
...individually. Although quicker and more sensitive than bacterial culture, PCR testing for strangles is still somewhat limited. PCR testing uses primers to target specific DNA sequences to determine an organism’s presence. Because of the genetic similarities between S. equi and S. zoo, some tests may not be able to differentiate between the two organisms and lead to false positives. Conversely, there are instances where the typically targeted sequ...
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TVMDL adds panel to test catalog to aid in the detection of strangles
...Multiple sample types can be submitted for this panel for testing by both PCR and culture. When submitting swabs, it is recommended to send duplicate swabs, one for PCR and a separate one for culture. Swabs submitted in bacterial transport media e.g., gel, Ames charcoal, etc. are not suitable for PCR testing due to inhibitory effects of the media. Clients are strongly encouraged to review the sample types and sampling requirements listed for this...
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Summary of Calf Diarrhea Multiplex Results from Texas Cattle, 2019-2023
...ailable epidemiological data from Texas cattle tested using this multiplex PCR. Animal Demographics & Geographic Locations Over the 4.5-year period for which data is available (March 2019 – October 2023), TVMDL performed 1,088 calf diarrhea multiplex PCR tests. Samples submitted for this test originated from 108 different counties in Texas. A dot density map representing the geographic distribution of samples is below; each dot represents a single...
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Bovine Abortion Caused by Leptospira spp.
...the organism in the serum from the cow or through antigen detection (e.g. PCR, rtPCR, etc.) testing of fetal tissues. The organism is shed in the urine of infected animals, thus personal protective equipment (PPE) should be utilized when working with cattle suspected to be infected in order to prevent human exposure. Additionally, minimizing exposure of susceptible animals to wildlife, rodents, and contaminated water sources, along with vaccinati...
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Bovine Abortion Diagnostics find BVD
...not detected via PCR. Bovine viral diarrhea virus (BVDV) was detected via PCR of the fetal tissues with a Ct value of 18.70. The cutoff Ct value for a positive BVDV PCR sample is <37.00. Bovine Comprehensive Abortion Serology was requested on the dam serum sample at the start of testing as well. Antibody testing was negative for Brucella abortus, Histophilus somni, Neospora caninum, and 5 serovars of Leptospira spp. There was a low (8) virus neut...
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Avian metapneumovirus (aMPV) tests added to TVMDL test catalog
...ay with results in 1 to 4 business days. Avian Metapneumovirus Type C (rRT-PCR) Test Code: 1612 Price: $28 Turnaround Time: This test is performed Monday through Friday with results in 1 to 4 business days. Avian Metapneumovirus Panel (rRT-PCR) Test Code: 1613 Price: $65 Turnaround Time: This test is performed Monday through Friday with results in 1 to 4 business days. For more information on TVMDL’s molecular diagnostics test offerings, visit tvm...
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Q fever abortion diagnosed at TVMDL
...all small ruminant abortions submitted to TVMDL be tested for Q fever by qPCR before any other test can be performed. If C. burnetiiq PCR results are positive, Select Agent regulations require that all fresh and fixed tissues must be destroyed properly and no other testing can be performed. Coxiella burnetii primarily affects goats, sheep, cattle, and humans. Dogs and cats can be infected by ingesting placentas and tissues of infected aborted fet...
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Lyme Disease Testing at TVMDL
...es (Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, Anaplasma phagocytophilum); Borrelia species (B. burgdorferi, B. hermsii, B. parkeri, B. turicatae); and Babesia species (B. gibsoni, B. canis, B. caballi). Test: Borrelia species (qPCR) Price: $30 Test: TickPath LayerPlex (qPCR) Price: $60 Borrelia burgdorferi depicted using IFA To learn more about TVMDL’s serology tests, contact Sandy Rodgers, Serology Section Head. For more...
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Porcine Circovirus type 2: A focus on respiratory disease
...t TVMDL to aid in the diagnosis of PCV-2 include Necropsy, Histopathology, PCR, virus isolation, and Serology. The recommended samples for PCR include intestine, fecal swab, lung or fetal tissue. For serology, an ELISA test is available, and serum is the required sample. To learn more about this case, contact Dr. Gabriel Gomez, veterinary pathologist, Dr. Pam Ferro, virology and molecular diagnostics section head in College Station, or Dr. Binu Ve...
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Swine Influenza Virus: A focus on the respiratory syndrome
...seen in the regenerative stage of the disease. For a definitive diagnosis, PCR on fresh tissue (trachea, lung, tracheal swab), serology (serum), immunohistochemistry on fixed tissue and virus isolation on fresh lung are tests available to help confirm an SIV infection. TVMDL currently offers necropsies and microscopic examination, virus isolation, serology, and PCR testing on fresh tissue. To learn more about this case or about Swine Influenza Vir...
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Listeriosis in a goat doe
...stically rewarding when submitted for cytology or pathogen detection via rtPCR if brain tissue is not available. Culture and rtPCR of CSF is less often rewarding compared to appropriate sections of affected fresh brain tissue. Figure 1: Photomicrograph depicting necrosuppurative encephalitis with microabscessation in the brainstem of the goat. Resources: Allen, Andrew L., Brad A. Goupil, and Beth A. Valentine. “A Retrospective Study of Brain Lesio...
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Epidemiology of feline hemoparasites in Texas
...ented as having 2 other “adult littermates” with similar symptoms. 17 cats were diagnosed by observing haemofelis in peripheral blood smears. One of these cats was also PCR positive. 15 cases were identified by PCR; 2 cats were still PCR positive 1 month after the initial diagnosis. To learn more about TVMDL’s feline hemoparasite testing, and other offerings, visit tvmdl.tamu.edu or call 1.888.646.5623....
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Infectious respiratory disease in a group of dogs
...dogs. In addition, canine parainfluenza virus type-2 RNA was detected by rtPCR (Ct values ranged from 24.5 to 29.5) in the lungs of the three dogs. Canine distemper virus, influenza A virus, canine adenovirus type 2, canine coronavirus, canine herpesvirus type 1 and Bordetella bronchiseptica nucleic acid were not detected by PCR in any of the dogs. Pneumonia in dogs is part of the canine infectious respiratory disease complex (CIRDC) and is a mult...
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TVMDL adds test to detect causative agent of ulcerative enteritis in avian species
...m bacteria are difficult to isolate with traditional culture methods, this PCR test allows for improved detection of the agent to aid in diagnosis of this disease. Test: Clostridium colinum (rtPCR) Test Code: 1556 Turnaround Time: This test is performed Tuesday through Friday at the College Station laboratory with results in one to four business days. Price: $25 For more information about TVMDL testing, visit tvmdl.tamu.edu or call one of the agen...
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Download Fee Schedule
...cine Parvovirus (HI) [Referral] 1 mL serum $12.21 8168 Porcine Parvovirus (PCR) [Referral] fetal tissue $35.00 8169 Porcine Parvovirus Type 2 (PCR) [Referral] One of the following: serum lung spleen $35.00 1519 Porcine Reproductive & Respiratory Syndrome Virus (ELISA) 1 mL serum $7.50 Canyon 1309 Porcine Reproductive & Respiratory Syndrome Virus (rtPCR) One or more of the following: 1-2 mL oral fluid, semen, serum 1-2 g fresh lung or fetal tissu...
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Small Animal Syndromic Diagnostic Plans
...tests TestSamplesTurnaround TimeSectionLabSchedule Campylobacter jejuni (rtPCR) 1-2 grams feces 1-3 days Molecular Diagnostics CAN CS TWRF Salmonella genus (rtPCR) 1-2 grams feces 1-4 days Molecular Diagnostics CS TWRF Schedule: M=Monday, T=Tuesday, W=Wednesday, R=Thursday, F=Friday, Sa=Saturday Feline Diarrhea Diagnostic Plan This plan was created to assist with the investigation of etiologic agents that may cause disease in cats. Recommended ini...
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Equine Protozoal Myeloencephalitis (EPM) Diagnosed in a Horse
...of the disease, though additional testing such as immunohistochemistry or PCR may be necessary to confirm the diagnosis. Rare cases of EPM are also caused by Neospora hughesi, which cannot be reliably differentiated histologically from Sarcocystis neurona. Immunohistochemistry and/or PCR are needed for ultimate differentiation. Besides EPM, there are many other differentials for neurologic disease in horses. One disease that should always be rule...
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Porcine Reproductive and Respiratory Syndrome: A focus on the respiratory syndrome
...boratory to aid in the diagnosis of PRRS include Necropsy, Histopathology, PCR, virus isolation, and Serology. The recommended samples for PCR testing include oral fluid, semen, serum, EDTA whole unclotted blood, lung, or fetal tissue. For serology, an ELISA and IFA test are available and serum is the required sample. To learn more about this case, contact Dr. Gomez, Veterinary Pathologist, Dr. Ferro, Microbiology Branch Chief, at the College Stat...
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CHI St. Joseph Health and Texas A&M Veterinary Medical Diagnostic Laboratory Collaborate for COVID-19 Testing
...) test, one of the two tests that are currently used to test for COVID-19. PCR swabs are currently collected at all CHI St. Joseph Health Emergency Centers as well as the South College Station Respiratory Clinic. Through this partnership, CHI St. Joseph Health staff can test up to 500 COVID-19 samples each day, allowing St. Joseph to greatly increase the number of tests performed daily in the community. “TVMDL is dedicated to protecting both anima...
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TVMDL, Physician’s Preferred Laboratory, Ltd. establish partnership to increase human COVID-19 testing
...e information needed to manage patient care, community health and safety.” PCR testing is the primary method used to detect COVID-19. PCR tests are used to detect the virus antigens. Antigens indicate the presence of a virus in its early stages of infection, even before antibodies form or symptoms are present. Dr. Andrew Hoot of Amarillo Pathology Group, LLP and Physicians Preferred Laboratory, Ltd. will serve as the medical director overseeing hu...
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Pseudorabies in a group of Texas hog-hunting dogs
...ncephalitis with lesions consistent with viral infection. The pseudorabies PCR results were positive. Pseudorabies is caused by suid herpesvirus 1. This virus has been eradicated from the commercial swine population in the United States, but the virus still circulates in feral swine populations. The prevalence of pseudorabies in Texas feral swine populations averages between 8-13% based on opportunistic sampling of adults done by the USDA/APHIS/Wi...
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How to create a comprehensive serology testing plan
...uation? Example: Following a positive serology test for leptospirosis, a rtPCR test on urine may hopefully identify and confirm the cause of the animal’s illness. Example: Following a positive serology test for Johne’s Disease, a rtPCR test on feces may yield a negative result because the organism is shed intermittently, even though the animal actually has Johne’s Disease. If a serology screening yields a negative result for antibodies to an agent...
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Texas Animal Health Commission updates trichomoniasis testing requirements
...amples must arrive at laboratory within 48 hours of collection In-Pouch (rtPCR) Ship in In-Pouch Incubate samples at 37ºC (98.6ºF) for 48 hours prior to testing Samples must arrive at laboratory within 96 hours of collection Pooling eligible for up to five samples Direct Smegma (rtPCR) Ship in tube containing 1.5 mL of PBS or sterile saline Samples must arrive at laboratory within 120 hours of collection Samples must be chilled from collection unt...
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TVMDL detects feline calicivirus in multi-cat household
...sis even with treatment (Dodd, J. personal communication, April 24, 2018). PCR technology can be used to identify oral calicivirus carriers. If you have any questions about this case or TVMDL’s Feline Calicivirus qPCR test please call Dr. Cheryl Maguire, veterinary diagnostician at 979-845-3414. For more information about TVMDL’s test catalog, visit tvmdl.tamu.edu. References 1. Gracis M. Inflammatory Oral Diseases in Cats. World Small Animal Asso...
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Epizootic hemorrhagic disease (EHD) in White-Tailed deer
...ical, hyperacute, acute, or chronic. EHDV nucleic acid can be detected by qPCR or EHDV can be isolated in the subclinical form, but clinical signs and macroscopic and microscopic lesions are absent and death does not occur. In the hyperacute form, sudden death is the outcome. Swelling of the head, neck, tongue, and eyelids can be observed in the acute form. In this form, there is fever, depression, anorexia, weakness, respiratory distress, ptyalis...
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Tick-Borne Relapsing Fever diagnosed in Brazos Valley dog
...along with a visibly decreased platelet count (Figure 1). Borrelia spp. rtPCR was then run and DNA from B. turicatae was detected with the cycle thresholds (Ct) being 21.6. According to TVMDL’s molecular laboratory, samples with a Ct value 1.0-28.0 indicate the presence of abundant amounts of target nucleic acid; samples with a Ct value of 28.1-35.0 indicate moderate amounts of target nucleic acid; samples with a Ct value of 35.1-40-45.0 indicate...
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Diagnostic Guidance: Postmortem Atlanto-occipital Cerebral Spinal Fluid Sampling in Large Animals
...Referral], Additive free (red top) sample collection tube: Microprotein, Electrolyte Profile, Bacterial Identification – Companion Animal (Aerobic & Anaerobic Culture) or Bacterial Identification – Livestock (Aerobic & Anaerobic Culture), Listeria monocytogenes (PCR) [Referral], West Nile Virus (rtPCR), Equine West Nile Virus IgM (ELISA), Eastern Equine Encephalitis Virus IgM (ELISA), Equine Encephalitis Panel (ELISA), Western Equine Encephalitis...
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Detection of Neospora caninum DNA in a mummified bovine fetus
...tozoa and viruses by standard methods. For the last two decades, real time PCR (rtPCR) has been the pillar in the diagnosis of infectious abortion in ruminants and it is of particular value in mummified fetuses where other ancillary tests are inadequate. References de la Concha-Bermejillo A, Romano JE: Pregnancy loss in cattle. Clinical Theriogenology; 2021, Submitted. de la Concha-Bermejillo A, Romano JE: The use of laboratory in pregnancy loss....
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Tick-borne disease in a dog with no known history of ticks
...ne Relapsing Fever, which was later confirmed with the TickPath Layerplex qPCR test at TVMDL. In the state of Texas, Tick-Borne Relapsing Fever (TBRF) is caused by Borrelia turicatae, which is spread by Ornithodoros turicata, a soft-bodied tick. These ticks are nocturnal, feeding on their hosts briefly at night. Therefore, it is not uncommon for dogs diagnosed with TBRF to lack a clinical history of tick infestations. The infection can cause letha...
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Mast Cell Tumor Prognostic Panel [Referral]
Evaluation of the biological behavior of a specific tumor entity. Includes IHC C-Kit, IHC Ki-67, PCR C-Kit Exon 8, PCR C-Kit Exon 11, and Agnor Special Staining.
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TVMDL retires Canine Distemper Virus (FA)
...e College Station laboratory at 1.888.646.5623....
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Testing Options for Anaplasma marginale in Cattle
...right-Giemsa stain, as well as Diff-Quick®, can be utilized for staining. qPCR (real time polymerase chain reaction) testing utilizes EDTA whole blood or fresh tissues to look for the DNA of the Anaplasma qPCR is best at detecting acute infections when bacteremia is still present, but may still be able to detect some infections after visual identification of the organism is no longer present on a blood smear. cELISA (competitive ELISA) testing is...
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Fetal loss and neonatal mortality due to Neospora caninum in a litter of German Shorthaired Pointers
...e not observed but fresh liver was tested via Canine Herpesvirus Type 1 (rtPCR) and CHV was not detected. Histology demonstrated necrotizing encephalitis, lymphoplasmacytic myocarditis, rhabdomyositis and pneumonia with protozoan cyst in the brain and intralesional tachyzoites in several tissues. These findings were consistent with systemic protozoal disease. Neospora caninum was detected in brain/heart tissue pool via (rtPCR) with a Ct Value of 2...
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Diagnostics of a disaster: Animal disease concerns during floods
...orms a microscopic agglutination test (MAT) and polymerase chain reaction (PCR) test for leptospirosis. The MAT can provide titers that indicate infection in the presence of compatible clinical signs. The PCR test can detect the presence of the organism in clinical specimens, which helps differentiate an infected animal from one that may be showing vaccine titers. Clostridial Diseases Herds can be vaccinated to protect against clostridial diseases...
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TVMDL pathologist contributes to three studies over cetacean pathology
...perspective of an unusual mortality event in Guiana dolphins, a real-time PCR to detect Cetacean morbillivirus, and histopathological differential diagnoses of Meningoencephalitis. Access each article at the links below. The Pathology of Cetacean Morbillivirus Infection and Comorbidities in Guiana Dolphins During an Unusual Mortality Event (Brazil, 2017–2018) A novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceans Histopathol...
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Cryptosporidium in a Gerenuk
...hoid hyperplasia, which was likely in response to the enteritis. Real-time PCR for cryptosporidium was used to confirm presence of cryptosporidium in feces of the calf. Cryptosporidium is a protozoal infection that affects a wide range of species (mammalian, avian, reptilian and piscine hosts) in addition to being zoonotic and affecting man. Animals obtain the protozoa through exposure to oocysts shed in the feces. Unapparent infections are common...
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Cryptosporidiosis in cattle
...hology), bacterial culture and sensitivity, and polymerase chain reaction (PCR) testing for viruses. There were no gross findings reported on the submittal form by the veterinarian. On microscopic examination, the major finding was the lesion in the small intestine. This lesion consisted of inflammation that was composed of a mixed-inflammatory cell population including macrophages, lymphocytes, plasma cells and neutrophils that were primarily wit...
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Tracking transboundary and emerging diseases: Newly funded project aims to use Next-Generation Sequencing to detect bovine diseases sooner
...thogen characterization. Currently, real-time polymerase chain reaction (rtPCR) assays are most widely used in diagnostic laboratories for rapid pathogen detection. These assays are useful, however, they are based on existing genomic information and may fail to detect emerging pathogens or variants of known pathogens. Additionally, rtPCR assays do not yield specific genomic information needed to track the source of disease outbreaks and differenti...
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TVMDL retires Mycoplasma gallisepticum (HI) and Mycoplasma synoviae (HI) tests
...tive, clients may request Mycoplasma gallisepticum & synoviae Multiplex (rtPCR). The rtPCR test is more sensitive and specific than the HI tests. Clients who still wish to use the HI method may submit samples to Poultry Research and Diagnostic Laboratory (PRDL) at Mississippi State University or the Georgia Poultry Laboratory Network (GPLN). For more information on TVMDL’s test offerings, visit tvmdl.tamu.edu or call one of the agency’s four labor...
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Direct smegma samples now accepted for trichomonas testing at TVMDL’s Canyon lab
...stic Laboratory (TVMDL) has expanded the Tritrichomonas foetus – Bovine (rtPCR) test offering. Clients interested in testing bovine samples for Tritrichomonas foetus can now submit direct smegma samples to the Canyon laboratory. Direct smegma samples should be submitted in a test tube with 1.5 mL of phosphate buffered saline and chilled during transit. To ensure samples arrive in a testable condition, smegma should be submitted to TVMDL within 96...
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Diagnostic guidance: Mycoplasma testing options
...nfertility, miscarriages, and weak offspring. These bacteria can also affect the mammary glands causing mastitis and poor milking. Though Mycoplasma species need special conditions to grow, TVMDL can culture for the bacteria from tissues or swabs. There are also polymerase chain reaction (PCR) tests that target the bacteria’s nucleic acid (DNA). Some of the PCR tests are specific for certain Mycoplasma species. Antibody tests are also available fo...
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Ehrlichia canis discovered in dog
...observed. Based on this finding, TVMDL’s Tick-Borne Pathogen Multiplex (rtPCR) test was performed and confirmed the presence of Ehrlichia canis. Ehrlichiosis in dogs is most commonly caused by infections with Ehrlichia canis and Ehrlichia ewingii. In the acute phases of the disease, morulae can be observed within WBCs during blood smear examination. Morulae are cytoplasmic vacuoles filled with bacteria. E. canis can be spread by the brown dog tic...
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Acute Toxoplasma gondii Infection Confirmed in a Canine
...tic Laboratory (TVMDL) in College Station for Toxoplasma gondii testing (rtPCR) which was positive with a Ct value of 15.70. Additional testing included Toxoplasma gondii IgG & IgM Panel IFA and Neospora caninum IFA on serum. IgG antibodies against Toxoplasma gondii were detected at a dilution of >=1:2048. IgM antibodies were not detected; nor were Neospora caninum antibodies detected. Toxoplasma gondii tachyzoites may be detected in various tissu...
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Histopathologic Diagnosis of Feline Herpesvirus
...aboratory (TVMDL). The most common laboratory methods of diagnosis include PCR and histopathology, while exposure to the virus can be demonstrated by measuring antibody response with the virus neutralization assay. This summer, two feline submissions demonstrated an array of respiratory histopathologic lesions that can be seen with herpesvirus infection. These cases were received two days apart but were completely unrelated and from two different...
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Díaz-Delgado publishes two articles over marine mammal pathology
...with Toxoplasma gondii. Immunohistochemical and polymerase chain reaction (PCR) analysis targeting a repetitive 529 bp DNA fragment of T. gondii confirmed toxoplasmosis in the liver, spleen, lung and lymph nodes. PCR-restriction fragment length polymorphism (RFLP) analysis using 11 markers identified a new non-archetypal genotype, ToxoDB-RFLP genotype #300. Further, the genotyping by microsatellite technique employed 15 markers and confirmed a uni...
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Diagnostic guidance: Tick-Borne Relapsing Fever
...blood films is considered diagnostic for TBRF in dogs. TVMDL also offers a PCR for TBRF Borrelia spp. Test: Borrelia spp. (rtPCR) Turnaround: This test is performed at the College Station laboratory with results in 1 to 3 business days. Price: $30 Treatment TBRF has been successfully treated in dogs with varying doses of doxycycline. On average, 6 mg/kg doxycycline by mouth every 12 hours for a minimum of three weeks appears to resolve clinical si...
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Babesia gibsoni (IFA) temporarily unavailable
...ho suspect Babesia gibsoni in canines may consider TVMDL’s Babesia spp. (rtPCR) as an alternative. Please note that clients testing for export purposes should review the country of interest’s export requirements to confirm PCR is an accepted testing method. If serologic testing is more appropriate, TVMDL can forward samples to Protatek Reference Laboratory in Mesa, Arizona. TVMDL is working with the slide manufacturer and will reorder more once th...
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Education Library: Bovine
...Keeping cattle healthy and hydrated during a drought BVDV: Sources and Test Guidance Definitive Diagnosis of Cryptosporidiosis Photosensitization: affecting cattle herds across the state Testing for Trichomonas Submitting Samples for Trich rtPCR Collecting samples for bovine trichomoniasis testing (video) How to submit samples for trich testing (video) Bovine Syndromic Diagnostic Plans Bovine Testing Guidance and Reference Material...
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Phelue Anderson, MS, PhD
...Dr. Anderson completed his doctorate in Poultry Science at Texas A&M University; previously he received a Master of Science in Animal Science from Prairie View A&M University. His graduate research focused on the use of PCR-based methodologies to characterize Salmonella serotypes of poultry origin. He is interested in bacteriology, molecular and serology testing of poultry diseases, as well as quality assurance programs for diagnostic work...
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West Nile Virus & Encephalitis
...ncephalitis Virus (EEE) with one confirmed case of WNV (brain sample by rt-PCR), 15 confirmed cases of WNV by IgM ELISA and one confirmed case of EEE by IgM ELISA. It is important to remember that these diseases have a seasonality. Our highest number of positive cases are usually observed in September and October (see figure 1.). There is still time to have your horse vaccinated for these preventable diseases. Mosquito control around your barn, st...
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IMHA in dogs secondary to Babesia gibsoni diagnosed at TVMDL
...consistent with Babesia gibsoni (see image), which were later confirmed by PCR. Babesia gibsoni is most commonly observed in pit bull terriers and greyhounds and infections are reported in several states throughout the US. IMHA is suspected when compatible CBC findings are observed, such as a marked regenerative anemia, hemoglobin:HCT mismatch, macroagglutination, and leukocytosis. Evaluation of a blood smear at a diagnostic laboratory is an essen...
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TVMDL Sections
...of both viral and bacterial pathogens using the polymerase chain reaction (PCR), which does not require culturing pathogens, thus eliminating the need to isolate or culture the pathogen, making the test results available quicker. A limited number of tests designed to detect genetic defects are also offered. The Necropsy Section performs necropsies on deceased animals to determine a probable cause of death or to collect specimens for additional tes...
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Infectious laryngotracheitis diagnosed in laying chicken
...large numbers of lymphocytes and plasma cells. Polymerase chain reaction (PCR) evaluation of tracheal swabs was positive for infectious laryngotracheitis virus. Infectious laryngotracheitis (ILT) is a respiratory tract infection of chickens caused by Gallid herpesvirus type 1 (GaHV-1). The virus may cause severe production losses due to mortality and/or decreased egg production. Severe epizootic forms of infection are characterized by signs of re...
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Cattle herds recently diagnosed with Johne’s Disease
...ease as an initial diagnosis. Follow up testing using the Johne’s Disease qPCR resulted in the detection of Mycobacterium avium subspecies paratuberculosis. This bacterium is the causative agent of Johne’s Disease, which was determined to the be primary cause of the weight loss and diarrhea in both cattle herds. In addition to testing for Johne’s Disease, a complete blood count and chemistry panel were requested for one case. Identification of Myc...
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Rhinosporidium diagnosed in Texas horses
...f diagnosis include cytology of nasal exudate to detect sporangiospores or PCR testing on fresh tissue. Currently, only histopathology and cytology are offered at the TVMDL. To learn more about this case, contact Dr. Gabriel Gomez, veterinary pathologist at the College Station laboratory. For more information on TVMDL’s test catalog, visit tvmdl.tamu.edu. Nasal Epithelium. The epithelium contains a pocket of inflammatory cells comprised primarily...
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Bovine CBC reveals concurrent BLV and Anaplasmosis
...sms consistent with Anaplasma marginale. Confirmatory testing included BLV PCR and Anaplasmosis ELISA. Bovine leukemia virus (BLV) is a retrovirus spread by contact with bodily fluids through biting flies, breeding, colostrum/milk, and certain management practices. Infections can results in asymptomatic carriers or severe clinical disease. Approximately 40% of infected cattle will develop persistent lymphocytosis, as seen in this case. Although nu...
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Neonatal Calf Health Issues
...ollowing tests routinely performed at TVMDL – bacterial culture, serology, PCR, virus isolation, and histopathology. Neonatal calf health issues can be complex and besides infectious disease or nutritional problems, may involve deficiencies in herd management. For this reason, please take advantage of TVMDL’s Veterinary Services for consultation on disease and management related conditions. In the area of livestock, Dr. Guy Sheppard at the College...
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Bacillary hemoglobinuria in feeder cattle
...iagnostic Laboratory (TVMDL) for culture, histopathology, Leptospira spp. qPCR, and histotoxic clostridium FA. Culture of the liver and kidney was negative for pathologic organisms. Histopathology revealed mild to moderate chronic fibrosing portal hepatitis with intralesional fluke eggs and adult flukes. Liver histopathology also showed acute centrilobular to midzonal hepatic necrosis with bile stasis. Molecular diagnostics on the urine, kidney, a...
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Strongylus vulgaris associated with liver damage and neurologic symptoms in a gelding
...re from fecal samples, a laborious and time consuming process. A real-time PCR assay has been developed and validated for the detection and semi-quantification of S. vulgaris eggs in fecal samples. Even more recently reported is the isolation of a S. vulgaris antigen, SvSXP, which may be used to detect targeted IgG antibodies against the parasite during the prepatent period. While generally viewed as a “historical” parasite, veterinarians should b...
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Abortion of a sheep fetus due to Cache Valley Fever Virus
...vities. Because of the type of lesions identified at the gross necropsy, a PCR test for Cache Valley Fever was performed with a positive result. The Cache Valley virus is in the order Bunyaviridae and genus Orthobunyavirus. It was first isolated from mosquitoes collected in Utah’s Cache Valley. This virus can cause outbreaks of spontaneous abortion and congenital malformations in sheep. In a non-pregnant adult animal, an infection may result in a...
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High mortality in a litter of puppies: Infection with canine herpesvirus
...chymotic renal hemorrhage. Available confirmatory diagnostic tests include PCR and histopathology, though viral inclusions are sometimes difficult to find histologically. Figure 1: Photo of kidney lesions at the time of necropsy. These kidneys have disseminated petechial to ecchymotic hemorrhages, a characteristic lesion of canine herpesvirus infection. Figure 2: Photomicrograph depicting renal necrosis (pink area in center) and hemorrhage. Inset:...
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Bacillary hemoglobinuria in a cow
...toxic clostridial specieswas positive for Clostridium novyi/hemolyticum. AqPCR test forLeptospirawas negative. Microscopic lesions in the liver consisted ofmultifocal to confluent areasof severe, acute coagulation necrosis characterized by hepatocyte swelling, cytoplasmic hypereosinophilia, karryorhexis,and pyknosis. The areas of hepatic necrosis were surrounded by a thick rim of degenerate neutrophils and necrotic debris. The gross and microscopi...
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Toxoplasmosis in a Herd of Kangaroos
...nd, most commonly in the heart and rarely in the brain and lung. T. gondii PCR was performed on the heart and brain from one kangaroo and was positive. This toxoplasmosis outbreak had a high mortality rate and left few survivors in the herd. Kangaroos and wallabies are highly susceptible to systemic toxoplasmosis and outbreaks can be devastating. In these species, infected animals often have sudden death though they may show neurological or respir...
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Cytoxic necrotizing factor 1 gene (CNF1) detected in E. coli isolated from a kitten lung
...was exudative and necrotizing, subacute, and severe, indicating a possible bacterial infection. Bacterial culture of the lung resulted in heavy growth of Escherichia coli. No other organisms were isolated. The cytotoxic necrotizing factor 1 gene (CNF1) was detected in this isolate by PCR testing. The significance of this finding remains unclear as there are only a few case reports discussing CNF1 detected in E. coli isolated from cats and dogs. F...
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Lymphoma diagnosed in a dog within an hour of fine needle aspiration via digital cytology service
...When the cytologic findings are equivocal, stained slides can be used for PCR for antigen receptor rearrangement (PARR). PARR testing can often confirm the diagnosis of lymphoma when cytologic examination is concerning but not definitive. For more information about this case, contact Clinical Pathology Section Head, Dr. Julie Piccione. To learn more about TVMDL’s testing services, visit tvmdl.tamu.edu or call 1.888.645.5623. Figure 1. Photomicrog...
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Specialty Services
...he art testing in areas such as mass spectrometry, classical and real-time PCR, nucleic acid sequencing, and rapid bacterial identification. We maintain a focus on developing and validating new diagnostic testing technologies and platforms to ensure our capabilities remain first class. Nontraditional Species TVMDL counts some of the largest wildlife preserves, zoos, and speciality animal operations as its clients. Staff are skilled in assisting wi...
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Ulcerative Enteritis discovered in bobwhite quail
...revealed no evidence of coccidia or nematodes. Polymerase chain reaction (PCR) testing of intestinal and liver samples demonstrated the presence of Clostridium colinum,the cause of ulcerative enteritis. Ulcerative enteritis is an acute bacterial infection of quail, but it also occurs in young chickens, turkeys, and other upland game birds. It is transmitted via the fecal-oral route under natural conditions; birds become infected by ingesting cont...
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Canine Enteropathogen Panel [Referral]
Canine panel includes PCR testing for Clostridium perfringens enterotoxin gene, net F toxin gene-C. perfringens, C. difficile, Campylobacter jejuni and coli, canine parvovirus, Salmonella spp., and IFA testing for Giardia and Cryptosporidium.
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Feline Enteropathogen Panel [Referral]
Feline panel includes includes PCR testing for Clostridium perfringens enterotoxin gene, net F toxin gene-C. perfringens, C. difficile, Campylobacter jejuni and coli, feline panleukopenia virus (FPV), Salmonella spp., Tritrichomonas foetus, and IFA testing for Giardia and Cryptosporidium.
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Unusual Manifestation of Papular Stomatitis in a Feedlot Steer
...) in the cell culture was compatible with bovine popular stomatitis virus. PCR analysis of viral nucleic acid was confirmatory for bovine papular stomatitis virus. Papular stomatitis is usually a mild disease in cattle and occurs worldwide. It is associated with proliferative skin lesions in the absence of systemic clinical disease and is most commonly seen as single or multiple cases in young cattle although outbreaks in dairy cattle are reported...
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Mycoplasmosis in Backyard Chicken Flocks
...al Diagnostic Laboratory (TVMDL). A real-time polymerase chain reaction (rtPCR) procedure is available that tests simultaneously for MG and MS. The test is conducted on tracheal and/or oropharyngeal swabs of clinically ill birds and is direct confirmation of the presence of the pathogen. A second indirect test can be performed on serum samples collected from birds that usually have been ill for a short period or have had clinical symptoms from whi...
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Malignant Catarrhal Fever in a 1-Year-Old Heifer
...d extensive ulceration of the bladder mucosa. Additional testing utilizing PCR technology for Malignant Catarrhal Fever (MCF) and Blue Tongue (BT) viruses followed. The BT virus was not detected, but MCF was positive with a CT value of 28.40. MCF is caused by viruses of the Macavirus genus that are in the Herpesviridae family (Gammaherpesvirinae subfamily). Two major groups of MCF viruses include one that primarily affects exotic even-toed ungulat...
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Factors involved in a Bovine Respiratory Disease Complex (BRDC) diagnosis
...erial organisms and molecular testing utilizing Polymerase Chain Reaction (PCR) technology are available at TVMDL to help identify the pathogens involved in the disease outbreak. In addition to culture, susceptibility testing can be performed on bacterial isolates to help guide the veterinarian in selection of antibiotics to be used for treatment of sick animals. Early diagnosis of pathogens involved in each outbreak of BRDC can be very helpful in...
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Contagious Ecthyma Diagnosed in a Young Goat
...ial infection. A section of this lip mass was submitted for parapoxvirus rtPCR. High levels of parapoxvirus DNA were detected, confirming the diagnosis of contagious ecthyma. This goat was also diagnosed with polioencephalomalacia as the cause of the neurologic disease. Contagious ecthyma is caused by a parapoxvirus and results in raised, crusty lesions which are most commonly found at mucocutaneous junctions, particularly around the mouth. In mor...
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Canine Coccidiosis
...ia microscopic examination due to their similar sizes (9-14 um). Real time PCR for T. gondii and N. caninum was performed on the fecal sample at TVMDL and no DNA was detected, leaving Hammondia heydorni as the only remaining identity for the parasite. Identification of the oocyst is important since T. gondii and N. caninum can cause severe disease in dogs while H. heydorniis largely considered clinically insignificant. Hammondia heydorni is a cocc...
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Neospora caninum Abortion in Cattle
...s on evidence of the organism in the fetal tissue by histopathology and/or PCR testing. The organism has an affinity for brain and heart tissue in the fetus, and these organs make the best specimens to use for diagnosis. No vaccine is available against this disease, and prevention requires restricting dogs’ access to cattle feedstuffs. For more information about TVMDL’s testing options for this disease and many others, visit tvmdl.tamu.edu or call...
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TVMDL experts working toward development of Newcastle disease assay thanks to grant
...nt opportunity for “Development and validation of a universal real-time RT-PCR assay to distinguish between virulent NDV and NDV of low-virulence and development of primers-probes bank for rapid distribution to field laboratories in response to a virulent NDV outbreak in the United States”. In January 2020, the proposal was awarded, and the planned research and development will commence in March 2020. This project is a collaborative effort between...
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Malignant Catarrhal Fever in a 1-year-old heifer
...d extensive ulceration of the bladder mucosa. Additional testing utilizing PCR technology for Malignant Catarrhal Fever (MCF) and Blue Tongue (BT) viruses followed. BT virus was not detected, but MCF was positive with a ct value of 28.40. MCF is caused by viruses of the Macavirus genus that are in the Herpesviridae family (Gammaherpesvirinae subfamily). Two major groups of MCF viruses include one that primarily affects exotic even-toed ungulates a...
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Johne’s Disease: How are cattle affected?
...antibodies to the organism, or by looking for the organism in feces using PCR or culture techniques. Since the incubation period for the disease is lengthy, testing young animals may result in false negative test results. No vaccine is available for the disease, so control methods involve removing positive animals from the herd and methods to reduce exposure to of young calves to the organism. For more information about this case, contact Dr. Guy...
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Mycoplasma synoviae (MS) infection in meat type chickens
...us exudate, and bloody/gelatinous material accumulated over the keel bone. PCR testing yielded positive results for MS. MS infection is a bacterial disease, which can affect the respiratory system, synovial lining of joints, tendon sheaths, and keel bursa as well. MS can be transmitted via respiratory secretions and trans-ovarially from infected breeders. MS strains exhibit variation in pathogenicity and some can cause severe economic losses to po...
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In-Pouch vs PBS for Trichomonas testing in bulls
...mples submitted in PBS also do not require incubation prior to running the PCR test, reducing turn around time for results by up to two days. As with almost everything, there are some possible disadvantages associated with the PBS test. Samples in PBS cannot be pooled, and culture for Trich is not an option for these samples. For a comprehensive guide to TVMDL’s Trich testing options, view this printable document. As a reminder, all bulls tested f...
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Hepatozoonosis in a dog
...ing dogs, rural dogs, and dogs that are mainly outdoor animals. There is a PCR test available, however finding the organisms on a peripheral blood smear may be accomplished by careful scrutiny and preparing a WBC buffy coat smear. In the past, many of these infected dogs were euthanized. There are reports of an effective treatment protocol that must be maintained for the dog’s life, but allows the pet to live a relatively normal existence. For mor...
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Molecular Diagnostics Section Overview
...ction Highlights High-volume testing section with ability to test 300-900 samples each day Advanced equipment with quick turnaround times Several screening tests for high consequence diseases and outbreak responses Overview of Molecular Diagnostics Services rtPCR testing for multiple diseases/conditions including: Trichomoniasis Avian Influenza Bovine Export Canine Respiratory Disease Feline Respiratory Disease College Station Leadership Interim S...
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Diagnostics of a disaster: Insect-borne disease concerns during floods
...is infected with EEE, WEE, or WNV. TVMDL also offers a molecular assay (rtPCR) for the detection of WNV. In addition, necropsy and histopathology can assist in the diagnosis of neurologic disease. Heartworm Disease Heartworm disease is caused by a parasitic filarial worm Dirofilaria immitis. This parasite is transmitted by mosquitoes. Dogs are primarily infected but this disease is also seen to a lesser extent in cats. As a result of extensive ra...
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Microfilaria identified in a penguin
...nary Medicine and Biomedical Sciences for further parasite identification. PCR results identified the parasite as belonging to the subfamily Splendidofilariina with the most likely genus being Paronchocerca sp. Splendidofilaria are a subspecies of avian filarial worms most of which are not pathogenic. Pathogenicity is even more rarely reported in association with microfilaria. The significance of this finding is unclear but ruling out Dirofilaria...
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Bovine thrombotic meningoncephalitis
...boratory’s (TVMDL) can detect H. somni nuclei acid in affected organs by rtPCR as part of the bovine bacterial respiratory panel or as an individual test. The differential diagnosis includes other neurological diseases of cattle including polioencephalomalacia, lead poisoning, salt poisoning, listeriosis, bovine herpesvirus encephalomyelitis, rabies and others. Treatment of affected cattle involves supportive care and use of antibiotics that will...
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TVMDL retires Bartonella heneslae IFA
...remained consistently low since last year. In addition, supplies needed to perform the test have become limited. As an alternative, the North Carolina State University – Vector Borne Disease Diagnostic Lab offers both IFA and PCR panels for various Bartonella species (including B. henselae). For more information about antibody testing at TVMDL, visit tvmdl.tamu.edu or call one of the agency’s full-service laboratories in College Station or Canyon....
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Aspergillosis in a Pacific Eider
...umnar conidial heads, and uniseriate phialides (Figures 3-4). Confirmatory PCR was not performed to identify the species. Eiders are highly susceptible to aspergillosis. Aspergillosis is common in other captive aquatic birds, especially penguins, and can also be seen in a variety of other avian species. In birds, most cases are caused by A. fumigatus. Recognized predisposing factors include poor ventilation, high environmental spore loads, high am...
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Anaplasmosis in cattle
...vary from very simple (direct examination of blood) to complex (ELISA and PCR). Veterinary assistance in suspected cases of anaplasmosis is very helpful in diagnosis, treatment, and control of this disease. For more information about testing for anaplasmosis, visit tvmdl.tamu.edu or call one of the agency’s full-service laboratories. Reference Capucille, Dawn J., Blackwell’s Five-Minute Veterinary Consult: Ruminant; Scott R.R. Haskell, DVM, edito...
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Schroeder named winner of 2020 Director’s Excellence Award
...ce a commercially available test kit to detect Trichomoniasis by real-time PCR. The commercialization of this kit would create a national standardization of testing methods and create consistency across several veterinary diagnostic laboratories. Most recently, Dr. Schroeder is commended for her assistance in the wake of the COVID-19 pandemic. She was instrumental in implementing both human and animal testing to detect SARS-CoV-2, the virus that c...
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Partnership between veterinary diagnostic laboratory and human healthcare providers recognized for response to COVID-19
...ghput equipment and laboratory space to perform polymerase chain reaction (PCR) testing. To make this partnership come to fruition, both entities had to overcome state and federal hurdles to receive authorization to perform testing on human samples at a lab traditionally used for veterinary diagnostics. Administrators from TVMDL and St. Joseph Health worked diligently to create a proposal that met the rigorous requirements of the Clinical Laborato...
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Enterotoxemia in sheep and goats
...stine and its contents. This can be followed by polymerase chain reaction (PCR) typing to determine the exact strain of the causative bacteria. For more information on enterotoxemia, contact Veterinary Diagnostician Dr. Guy Sheppard or Veterinary Pathologist Dr. Randi Gold. Learn more about TVMDL’s test offerings, by visiting tvmdl.tamu.edu. Figure 1: The intestines contain a segment of bowel that is dark red to purple (marked necrohemorrhagic ent...
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Listeriosis in Cattle
...and thromboembolic meningoencephalitis. Tests available include culture or PCR testing of non-fixed brain tissue or cerebrospinal fluid as well as formalin fixed brain tissue for histopathological examination. Since rabies testing is also frequently requested in these cases, please inform the TVMDL if a rabies test is being performed at the Department of State Health Services laboratory concurrently with the TVMDL testing. For more information on...
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Diagnostic Guidance: Bovine Respiratory Disease (BRD) Complex
...to determine sensitivity to antibiotics, while Polymerase Chain Reaction (PCR) testing methods can be used to quickly detect viral or bacterial BRD pathogens. Serological tests are also available to assess antibody levels to various BRD pathogens as an aid in diagnosis. Veterinary diagnosticians at both the College Station and Canyon laboratories are happy to consult with producers and veterinarians about their specific situations and to assist i...
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Pinkeye in cattle
...TVMDL that can help with diagnosis are cultures and sensitivity as well as PCR testing for Mycoplasma spp. and IBR. Prevention is aimed at controlling flies and providing uncrowded shady areas for the cattle. Commercial vaccines are available and can be helpful in problem herds. For more information on testing cattle for pinkeye, contact one of TVMDL’s laboratories in College Station or Canyon. Reference Carr, M.N. and Occhipinti, L.P., Blackwell’...
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Cytauxzoon felis infection in two cats
...t-laden macrophages are even rarer in circulation/blood smear examination. PCR testing can be performed to catch early infections and to potentially differentiate between other protozoan organisms. The disease in domestic cats typically progresses rapidly and death within 36 hours is common; however, treatment with supportive care and anti-protozoal drugs have been successful. Additionally, subclinical infections have been recognized in the last f...
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The threat of canine herpesvirus to pregnant bitches and neonate puppies
...s. Moreover, active infections can be assessed through virus isolation and PCR assays performed on nasal, ocular, and genital secretions, as well as on tissues from aborted fetuses or deceased puppies. Importantly, necropsy and histopathological evaluation can provide valuable insights. Preventive measures for CHV require an interdisciplinary effort. With no vaccine currently available for CHV in the United States, it is critical to mitigate viral...
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...nebacterium pseudotubercolosis How to Safely Package a Sample for Shipping at TVMDL Fumonisin Facts Pigeon Fever Submitting Samples for Trich rtPCR Tube Reference Chart How to Assemble a Necropsy Field Kit...
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TVMDL Fee Schedule
Download Full Fee Schedule (PDF) Accession Fee: $8.00 for all clients per accession. Accession fees apply to each accession/case, not each specimen. An accession is defined samples from the same animal/herd/flock, originating from a single animal owner, submitted for the same condition, collected at the same time. This allows clients to receive bulk testing of same species (from the same owner) under one accession. Poultry submissions requesting...
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Bluetongue Virus Isolation (Egg Inoculation)
Isolation of bluetongue virus (BTV) utilizing the intravenous (IV) inoculation of 10-12 day old chicken eggs.
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Virus Isolation (Egg Inoculation)
General procedure utilized for the isolation and identification of viruses.
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Urine Protein Creatinine Ratio
Measurement of urine microprotein and creatinine by automated analyzer. Includes calculation of urine protein creatinine ratio (UPC).
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Epizootic Hemorrhagic Disease Virus (Cell Culture)
Isolation and identification of epizootic hemorrhagic disease virus (EHD) using cell culture methods.
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Canine Distemper Virus Typing [Referral]
Differentiation between vaccine and wild-type strains of CDV using targeted sequencing.
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Avian Reovirus Sequencing & Genotyping
Sequencing and genotyping of avian reovirus isolates.