One or more of the following: tracheal swab, oropharyngeal swab, cloacal swab, 1-2 mL tracheal wash, 1 g fresh trachea.
Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid and discard the swab. Swabs in brain heart infusion (BHI) broth or viral transport media are preferred. Saline may be used if necessary but swabs should not be sent dry (virus is undetectable after 24 hours on a dry swab). For poultry, pooling up to 11 oropharyngeal or trachea swabs per tube is permitted (allow for 500 ul of media per swab in transport tube).