One or more of the following: tracheal swab, oropharyngeal swab, cloacal swab, 1-2 mL tracheal wash, 1.0 g fresh tracheal tissue
Swabs in brain heart infusion (BHI) broth or viral transport media are preferred. Saline may be used if necessary but swabs should not be sent dry (virus is undetectable after 24 hours on a dry swab). For poultry, pooling up to 11 oropharyngeal or trachea swabs per tube is permitted (allow for 500ul of media per swab in transport tube).
Swabs in broth or viral transport media
Label all samples. Ship overnight to arrive within 48 hours of collection. Ship on ice.
Swabs must be removed from media. Swirl each swab in the liquid, press tip of swab on side of tube to release remaining liquid and discard the swab. After the last swab recap tube and send the tube with media on ice by overnight courier.