Effective November 1, the Texas A&M Veterinary Medical Diagnostic Laboratory (TVMDL) began accepting samples for the new Tritrichomonas foetus (Tfoe) Direct qPCR test.
The Tfoe Direct qPCR test has increased sensitivity which aids in the detection of Tfoe infected bulls. However, due to the sensitive nature of molecular based tests (qPCR) practitioners must follow proper collection techniques to reduce the possibility of cross contamination which insures a more accurate test result.
Practices such as changing gloves between bulls, keeping samples out of direct sunlight, keeping samples cold after collection and during transit, and shipping samples to the laboratory as soon after collection as possible will help in determining an accurate diagnosis. Ideally, TVMDL should receive samples within 48 hours of collection, however Texas Animal Health Commission (TAHC) regulations do allow samples to arrive at the lab within 96 hours of collection.
In addition to greater sensitivity, the Tfoe Direct qPCR test offers a quicker turnaround time since sample incubation is not required. Compared to other TAHC approved Trichomoniasis tests, the Tfoe Direct qPCR method does not require the use of an InPouch. If a Tfoe culture is required the sample must be submitted in an InPouch and must arrive at the lab within 48 hours of collection
Test: Tritrichomonas foetus – Bovine (qPCR)
Specimen: Direct smegma in 1.5 mL of PBS (phosphate buffered saline)
Turnaround: This test is run at the College Station lab Monday through Friday with results in 2 to 4 business days.
Learn more about TVMDL and view our entire test catalog by visiting tvmdl.tamu.edu. For questions about the Direct qPCR test, call the College Station laboratory at 1.888.646.5623.